View source for 2kdy


==NMR structure of LP2086-B01==
<StructureSection load='2kdy' size='340' side='right'caption='[[2kdy]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2kdy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KDY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KDY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kdy OCA], [https://pdbe.org/2kdy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kdy RCSB], [https://www.ebi.ac.uk/pdbsum/2kdy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kdy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q6VRY4_NEIME Q6VRY4_NEIME] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kd/2kdy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kdy ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
LP2086 is a family of outer membrane lipoproteins from Neisseria meningitidis, which elicits bactericidal antibodies and are currently undergoing human clinical trials in a bivalent formulation where each antigen represents one of the two known LP2086 subfamilies. Here we report the NMR structure of the recombinant LP2086 variant B01, a representative of the LP2086 subfamily B. The structure reveals a novel fold composed of two domains: a "taco-shaped" N-terminal beta-sheet and a C-terminal beta-barrel connected by a linker. The structure in micellar solution is consistent with a model of LP2086 anchored to the outer membrane bilayer through its lipidated N terminus. A long flexible chain connects the folded part of the protein to the lipid anchor and acts as spacer, making both domains accessible to the host immune system. Antibodies broadly reactive against members from both subfamilies have been mapped to the N terminus. A surface of subfamily-defining residues was identified on one face of the protein, offering an explanation for the induction of subfamily-specific bactericidal antibodies.

Structural Basis for the Immunogenic Properties of the Meningococcal Vaccine Candidate LP2086.,Mascioni A, Bentley BE, Camarda R, Dilts DA, Fink P, Gusarova V, Hoiseth SK, Jacob J, Lin SL, Malakian K, McNeil LK, Mininni T, Moy F, Murphy E, Novikova E, Sigethy S, Wen Y, Zlotnick GW, Tsao DH J Biol Chem. 2009 Mar 27;284(13):8738-46. Epub 2008 Dec 22. PMID:19103601<ref>PMID:19103601</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2kdy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Neisseria meningitidis]]
[[Category: Bentley BE]]
[[Category: Camarda R]]
[[Category: Dilts DA]]
[[Category: Fink P]]
[[Category: Gusarova V]]
[[Category: Hoiseth S]]
[[Category: Jacob J]]
[[Category: Lin SL]]
[[Category: Malakian K]]
[[Category: Mascioni A]]
[[Category: McNeil LK]]
[[Category: Mininni T]]
[[Category: Moy F]]
[[Category: Murphy E]]
[[Category: Novikova E]]
[[Category: Sigethy S]]
[[Category: Tsao DHH]]
[[Category: Wen Y]]
[[Category: Zlotnick GW]]