View source for 2kj1


==cytoplasmic domain structure of BM2 proton channel from influenza B virus==
<StructureSection load='2kj1' size='340' side='right'caption='[[2kj1]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2kj1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_B_virus Influenza B virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KJ1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kj1 OCA], [https://pdbe.org/2kj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kj1 RCSB], [https://www.ebi.ac.uk/pdbsum/2kj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kj1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9WD75_9INFB Q9WD75_9INFB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Influenza B virus contains an integral membrane protein, BM2, that oligomerizes in the viral membrane to form a pH-activated proton channel. Here we report the solution structures of both the membrane-embedded channel domain and the cytoplasmic domain of BM2. The channel domain assumes a left-handed coiled-coil tetramer formation with a helical packing angle of -37 degrees to form a polar pore in the membrane for conducting ions. Mutagenesis and proton flux experiments identified residues involved in proton relay and suggest a mechanism of proton conductance. The cytoplasmic domain of BM2 also forms a coiled-coil tetramer. It has a bipolar charge distribution, in which a negatively charged region interacts specifically with the M1 matrix protein that is involved in packaging the genome in the virion. This interaction suggests BM2 also recruits matrix proteins to the cell surface during virus budding, making BM2 an unusual membrane protein with the dual roles of conducting ions and recruiting proteins to the membrane.

Solution structure and functional analysis of the influenza B proton channel.,Wang J, Pielak RM, McClintock MA, Chou JJ Nat Struct Mol Biol. 2009 Dec;16(12):1267-71. Epub 2009 Nov 8. PMID:19898475<ref>PMID:19898475</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2kj1" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ion channels 3D structures|Ion channels 3D structures]]
*[[M2 protein|M2 protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Influenza B virus]]
[[Category: Large Structures]]
[[Category: Chou J]]
[[Category: McClintock M]]
[[Category: Pielak R]]
[[Category: Wang J]]