View source for 2klo


==Structure of the Cdt1 C-terminal domain==
<StructureSection load='2klo' size='340' side='right'caption='[[2klo]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2klo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KLO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2klo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2klo OCA], [https://pdbe.org/2klo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2klo RCSB], [https://www.ebi.ac.uk/pdbsum/2klo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2klo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CDT1_MOUSE CDT1_MOUSE] Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.<ref>PMID:11850834</ref> <ref>PMID:12192004</ref> <ref>PMID:14993212</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/2klo_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2klo ConSurf].
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== Publication Abstract from PubMed ==
In eukaryotic replication licensing, Cdt1 plays a key role by recruiting the MCM2-7 complex onto the origin of chromosome. The C-terminal domain of mouse Cdt1 (mCdt1C), the most conserved region in Cdt1, is essential for licensing and directly interacts with the MCM2-7 complex. We have determined the structures of mCdt1CS (mCdt1C_small; residues 452 to 557) and mCdt1CL (mCdt1C_large; residues 420 to 557) using X-ray crystallography and solution NMR spectroscopy, respectively. While the N-terminal 31 residues of mCdt1CL form a flexible loop with a short helix near the middle, the rest of mCdt1C folds into a winged helix structure. Together with the middle domain of mouse Cdt1 (mCdt1M, residues 172-368), this study reveals that Cdt1 is formed with a tandem repeat of the winged helix domain. The winged helix fold is also conserved in other licensing factors including archaeal ORC and Cdc6, which supports an idea that these replication initiators may have evolved from a common ancestor. Based on the structure of mCdt1C, in conjunction with the biochemical analysis, we propose a binding site for the MCM complex within the mCdt1C.

Structure of the Cdt1 C-terminal domain: Conservation of the winged helix fold in replication licensing factors.,Khayrutdinov BI, Bae WJ, Yun YM, Lee JH, Tsuyama T, Kim JJ, Hwang E, Ryu KS, Cheong HK, Cheong C, Ko JS, Enomoto T, Karplus PA, Guntert P, Tada S, Jeon YH, Cho Y Protein Sci. 2009 Aug 31. PMID:19722278<ref>PMID:19722278</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2klo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Bae WJ]]
[[Category: Cheong C]]
[[Category: Cheong H-K]]
[[Category: Cho Y]]
[[Category: Guntert P]]
[[Category: Hwang E]]
[[Category: Jeon YH]]
[[Category: Karplus PA]]
[[Category: Khayrutdinov BI]]
[[Category: Kim JJ]]
[[Category: Ryu K-S]]
[[Category: Tada S]]
[[Category: Tsuyama T]]
[[Category: Yun YM]]