View source for 2ksy


==Solution nmr structure of sensory rhodopsin II==
<StructureSection load='2ksy' size='340' side='right'caption='[[2ksy]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ksy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 30 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ksy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksy OCA], [https://pdbe.org/2ksy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ksy RCSB], [https://www.ebi.ac.uk/pdbsum/2ksy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ksy ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2ksy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ksy ConSurf].
<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
Seven-helix membrane proteins represent a challenge for structural biology. Here we report the first NMR structure determination of a detergent-solubilized seven-helix transmembrane (7TM) protein, the phototaxis receptor sensory rhodopsin II (pSRII) from Natronomonas pharaonis, as a proof of principle. The overall quality of the structure ensemble is good (backbone r.m.s. deviation of 0.48 A) and agrees well with previously determined X-ray structures. Furthermore, measurements in more native-like small phospholipid bicelles indicate that the protein structure is the same as in detergent micelles, suggesting that environment-specific effects are minimal when using mild detergents. We use our case study as a platform to discuss the feasibility of similar solution NMR studies for other 7TM proteins, including members of the family of G protein-coupled receptors.

Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy.,Gautier A, Mott HR, Bostock MJ, Kirkpatrick JP, Nietlispach D Nat Struct Mol Biol. 2010 Jun;17(6):768-74. Epub 2010 May 30. PMID:20512150<ref>PMID:20512150</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2ksy" style="background-color:#fffaf0;"></div>

==See Also==
*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Natronomonas pharaonis]]
[[Category: Bostock MJ]]
[[Category: Gautier A]]
[[Category: Kirkpatrick JP]]
[[Category: Mott HR]]
[[Category: Nietlispach D]]