View source for 2l0e


==Structural and functional analysis of tm vi of the nhe1 isoform of the na+/h+ exchanger==
<StructureSection load='2l0e' size='340' side='right'caption='[[2l0e]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2l0e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L0E FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 40 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0e OCA], [https://pdbe.org/2l0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l0e RCSB], [https://www.ebi.ac.uk/pdbsum/2l0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/2l0e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2l0e ConSurf].
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== Publication Abstract from PubMed ==
The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein. It resides on the plasma membrane of cells and regulates intracellular pH in mammals by extruding an intracellular H+ in exchange for one extracellular Na+. We characterized structural and functional aspects of the transmembrane (TM) segment VI (residues 227-249) by using cysteine scanning mutagenesis and high resolution NMR. Each residue of TM VI was mutated to cysteine in the background of the cysteineless NHE1 protein and the sensitivity to water soluble sulfhydryl reactive compounds MTSET ([2- (Trimethylammonium) Ethyl]Methanethiosulfonate) and MTSES ([2-Sulfonatoethyl]Methanethiosulfonate) was determined for those residues with significant activity remaining. Three residues were essentially inactive when mutated to Cys, Asp238, Pro239 and Glu247. Of the remaining residues, proteins with the mutations N227C, I233C, and L243C were strongly inhibited by MTSET while amino acids Phe230, Gly231, Aal236, Val237, Ala244, Val245 and Glu248 were partially inhibited by MTSET. MTSES did not affect activity of the mutant NHE1 proteins. The structure of a peptide representing TM VI was determined using high resolution NMR spectroscopy in dodecylphosphocholine (DPC) micelles. TM VI contains two helical regions oriented at an approximate right angle to each other (residues 229-236 and 239-250) surrounding a central unwound region. This structure bares a resemblance to TM IV of the E. coli protein NhaA. The results demonstrate that TM VI of NHE1 is a discontinuous pore lining helix with residues Asn227, Ile233 and Leu243 lining the translocation pore.

Structural and functional analysis of transmembrane VI of the NHE1 isoform of the Na+/H+ exchanger.,Tzeng J, Lee BL, Sykes BD, Fliegel L J Biol Chem. 2010 Sep 15. PMID:20843797<ref>PMID:20843797</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2l0e" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Fliegel L]]
[[Category: Lee BL]]
[[Category: Sykes BD]]
[[Category: Tzeng J]]