View source for 2lkz


==Solution structure of the second RRM domain of RBM5==
<StructureSection load='2lkz' size='340' side='right'caption='[[2lkz]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lkz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkz OCA], [https://pdbe.org/2lkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkz RCSB], [https://www.ebi.ac.uk/pdbsum/2lkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RBM5_HUMAN RBM5_HUMAN] Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.<ref>PMID:10949932</ref> <ref>PMID:12207175</ref> <ref>PMID:12581154</ref> <ref>PMID:15192330</ref> <ref>PMID:16585163</ref> <ref>PMID:18851835</ref> <ref>PMID:18840686</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The RNA binding motif protein 5 (RBM5), also known as LUCA15 or H37, containing two RNA recognition motifs, is a component of the spliceosome A complex. Previously, it has been reported that RBM5 bound to a U/C-rich sequence upstream of In100 element at intron 9 of caspase2 pre-mRNA that enhanced the formation of proapoptotic caspase-2L isoform. In present studies, we solved the solution structure of the RBM5 RRM2 core domain and characterized its unusual binding capability for different RNA sequences. We found that the RBM5 RRM2 could preferentially bind to both CU rich and GA rich sequences with affinity in 10-5 molar range. Further NMR experiments revealed that the dual RNA molecules could be accommodated on almost the same region of the protein's beta-sheet surface and both the N- and C-terminal regions of the protein were involved in the recognition. Our studies provide evidence for RBM5 sequence specific interaction with cis-acting element in pre-mRNA to regulate alternative splicing.

Solution structure of the second RRM domain of RBM5 and its unusual binding characters for different RNA targets.,Song Z, Wu P, Ji P, Zhang J, Gong Q, Wu J, Shi Y Biochemistry. 2012 Jul 27. PMID:22839758<ref>PMID:22839758</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2lkz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Shi Y]]
[[Category: Song Z]]
[[Category: Wu J]]
[[Category: Wu P]]
[[Category: Zhang J]]