View source for 2lmk


==Solution Structure of Mouse Pheromone ESP1==
<StructureSection load='2lmk' size='340' side='right'caption='[[2lmk]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lmk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LMK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lmk OCA], [https://pdbe.org/2lmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lmk RCSB], [https://www.ebi.ac.uk/pdbsum/2lmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lmk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ESP1_MOUSE ESP1_MOUSE] Male-specific phermone which is recognized by the Vmn2r116/V2rp5 receptor in the vomeronasal organ (VNO) and enhances female sexual receptive behavior (lordosis) upon male mounting, resulting in successful copulation.<ref>PMID:16208374</ref> <ref>PMID:17935991</ref> <ref>PMID:20596023</ref> <ref>PMID:23576433</ref> <ref>PMID:17935991</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Exocrine gland-secreting peptide 1 (ESP1) is a sex pheromone that is released in male mouse tear fluids and enhances female sexual receptive behavior. ESP1 is selectively recognized by a specific class-C G-protein-coupled receptor (GPCR), V2Rp5, among the hundreds of receptors expressed in vomeronasal sensory neurons (VSNs). The specific sensing mechanism of the mammalian peptide pheromone by the class-C GPCR remains to be elucidated. Here we identified the minimal functional region needed to retain VSNs-stimulating activity in ESP1, and determined its three-dimensional structure, which adopts a helical fold stabilized by an intramolecular disulfide bridge with extensive charged patches. We then identified the amino acids involved in the activation of VSNs by a structure-based mutational analysis, revealing that the highly charged surface is crucial for the ESP1 activity. We also demonstrated that ESP1 specifically bound to an extracellular region of V2Rp5 by an in vitro pull-down assay. Based on homology modeling of V2Rp5 using the structure of the metabotropic glutamate receptor, we constructed a docking model of the ESP1-V2Rp5 complex in which the binding interface exhibited good electrostatic complementarity. These experimental results, supported by the molecular docking simulations, reveal that charge-charge interactions determine the specificity of ESP1-binding to V2Rp5 in the large extracellular region characteristic of class-C GPCRs. The present study provides insights into the structural basis for the narrowly-tuned sensing of mammalian peptide pheromones by class-C GPCRs.

Structure of the Mouse Sex Peptide Pheromone ESP1 Reveals a Molecular Basis for Specific Binding to the Class-C G-Protein-Coupled Vomeronasal Receptor.,Yoshinaga S, Sato T, Hirakane M, Esaki K, Hamaguchi T, Haga-Yamanaka S, Tsunoda M, Kimoto H, Shimada I, Touhara K, Terasawa H J Biol Chem. 2013 Apr 10. PMID:23576433<ref>PMID:23576433</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2lmk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Esaki K]]
[[Category: Haga-Yamanaka S]]
[[Category: Hamaguchi T]]
[[Category: Hirakane M]]
[[Category: Kimoto H]]
[[Category: Sato T]]
[[Category: Shimada I]]
[[Category: Terasawa H]]
[[Category: Touhara K]]
[[Category: Tsunoda M]]
[[Category: Yoshinaga S]]