View source for 2lml


==Solution NMR structure of holo acyl carrier protein from geobacter Metallireducens refined with nh rdcs, Northeast Structural Genomics consortium target gmr141==
<StructureSection load='2lml' size='340' side='right'caption='[[2lml]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lml]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_metallireducens_GS-15 Geobacter metallireducens GS-15]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2kwm 2kwm], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2kjs 2kjs] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2kci 2kci]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LML FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lml OCA], [https://pdbe.org/2lml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lml RCSB], [https://www.ebi.ac.uk/pdbsum/2lml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lml ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q39T60_GEOMG Q39T60_GEOMG] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
GmACP3 from Geobacter metallireducens is a specialized acyl carrier protein (ACP) whose gene, gmet_2339, is located near genes encoding many proteins involved in lipopolysaccharide (LPS) biosynthesis, indicating a likely function for GmACP3 in LPS production. By overexpression in Escherichia coli, about 50% holo-GmACP3 and 50% apo-GmACP3 were obtained. Apo-GmACP3 exhibited slow precipitation and non-monomeric behavior by (15)N NMR relaxation measurements. Addition of 4'-phosphopantetheine (4'-PP) via enzymatic conversion by E. coli holo-ACP synthase resulted in stable &gt;95% holo-GmACP3 that was characterized as monomeric by (15)N relaxation measurements and had no indication of conformational exchange. We have determined a high-resolution solution structure of holo-GmACP3 by standard NMR methods, including refinement with two sets of NH residual dipolar couplings, allowing for a detailed structural analysis of the interactions between 4'-PP and GmACP3. Whereas the overall four helix bundle topology is similar to previously solved ACP structures, this structure has unique characteristics, including an ordered 4'-PP conformation that places the thiol at the entrance to a central hydrophobic cavity near a conserved hydrogen-bonded Trp-His pair. These residues are part of a conserved WDSLxH/N motif found in GmACP3 and its orthologs. The helix locations and the large hydrophobic cavity are more similar to medium- and long-chain acyl-ACPs than to other apo- and holo-ACP structures. Taken together, structural characterization along with bioinformatic analysis of nearby genes suggests that GmACP3 is involved in lipid A acylation, possibly by atypical long-chain hydroxy fatty acids, and potentially is involved in synthesis of secondary metabolites.

Solution structure of 4'-phosphopantetheine - GmACP3 from Geobacter metallireducens: a specialized acyl carrier protein with atypical structural features and a putative role in lipopolysaccharide biosynthesis.,Ramelot TA, Smola MJ, Lee HW, Ciccosanti C, Hamilton K, Acton TB, Xiao R, Everett JK, Prestegard JH, Montelione GT, Kennedy MA Biochemistry. 2011 Mar 8;50(9):1442-53. Epub 2011 Feb 14. PMID:21235239<ref>PMID:21235239</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2lml" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacter metallireducens GS-15]]
[[Category: Large Structures]]
[[Category: Acton TB]]
[[Category: Ciccosanti C]]
[[Category: Everett JK]]
[[Category: Foote EL]]
[[Category: Hamilton K]]
[[Category: Kennedy MA]]
[[Category: Lee H]]
[[Category: Montelione GT]]
[[Category: Nair R]]
[[Category: Prestegard JH]]
[[Category: Ramelot TA]]
[[Category: Rost B]]
[[Category: Smola MJ]]
[[Category: Swapna G]]
[[Category: Xiao R]]
[[Category: Zhao L]]