View source for 2lo0


==Solution structure of the Get5 carboxyl domain from A. fumigatus==
<StructureSection load='2lo0' size='340' side='right'caption='[[2lo0]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lo0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LO0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lo0 OCA], [https://pdbe.org/2lo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lo0 RCSB], [https://www.ebi.ac.uk/pdbsum/2lo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lo0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q4WE50_ASPFU Q4WE50_ASPFU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Tail-anchored trans-membrane proteins are targeted to membranes post-translationally. The proteins Get4 and Get5 form an obligate complex that catalyzes the transfer of tail-anchored proteins destined to the endoplasmic reticulum from Sgt2 to the cytosolic targeting factor Get3. Get5 forms a homodimer mediated by its carboxyl domain. We show here that a conserved motif exists within the carboxyl domain. A high resolution crystal structure and solution NMR structures of this motif reveal a novel and stable helical dimerization domain. We additionally determined a solution NMR structure of a divergent fungal homolog, and comparison of these structures allows annotation of specific stabilizing interactions. Using solution x-ray scattering and the structures of all folded domains, we present a model of the full-length Get4/Get5 complex.

Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex.,Chartron JW, Vandervelde DG, Rao M, Clemons WM Jr J Biol Chem. 2012 Mar 9;287(11):8310-7. Epub 2012 Jan 17. PMID:22262836<ref>PMID:22262836</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2lo0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aspergillus fumigatus Af293]]
[[Category: Large Structures]]
[[Category: Chartron JW]]
[[Category: Clemons Jr WM]]
[[Category: Rao M]]
[[Category: Vandervelde DG]]