View source for 2los


==Backbone structure of human membrane protein TMEM14C==
<StructureSection load='2los' size='340' side='right'caption='[[2los]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2los]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LOS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2los FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2los OCA], [https://pdbe.org/2los PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2los RCSB], [https://www.ebi.ac.uk/pdbsum/2los PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2los ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TM14C_HUMAN TM14C_HUMAN] Required for normal heme biosynthesis (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Although nearly half of today's major pharmaceutical drugs target human integral membrane proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank, largely owing to inefficiencies in protein production. Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spectroscopy with systematically labeled proteins produced via cell-free expression. We report new backbone structures of six hIMPs, solved in only 18 months from 15 initial targets. Application of our protocols to an additional 135 hIMPs with molecular weight &lt;30 kDa yielded 38 hIMPs suitable for structural characterization by solution NMR spectroscopy without additional optimization.

Facile backbone structure determination of human membrane proteins by NMR spectroscopy.,Klammt C, Maslennikov I, Bayrhuber M, Eichmann C, Vajpai N, Chiu EJ, Blain KY, Esquivies L, Kwon JH, Balana B, Pieper U, Sali A, Slesinger PA, Kwiatkowski W, Riek R, Choe S Nat Methods. 2012 May 20;9(8):834-9. doi: 10.1038/nmeth.2033. PMID:22609626<ref>PMID:22609626</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2los" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Choe S]]
[[Category: Klammt C]]
[[Category: Maslennikov I]]
[[Category: Riek R]]
[[Category: Vajpai N]]