View source for 2lp0


==The solution structure of homeodomain-protein complex==
<StructureSection load='2lp0' size='340' side='right'caption='[[2lp0]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lp0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LP0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lp0 OCA], [https://pdbe.org/2lp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lp0 RCSB], [https://www.ebi.ac.uk/pdbsum/2lp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lp0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HXC9_HUMAN HXC9_HUMAN] Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
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== Publication Abstract from PubMed ==
Homeodomain-containing transcription factors play a fundamental role in the regulation of numerous developmental and cellular processes. Their multiple regulatory functions are accomplished through context-dependent inputs of target DNA sequences and collaborating protein partners. Previous studies have well established the sequence-specific DNA binding to homeodomains; however, little is known about how protein partners regulate their functions through targeting homeodomains. Here we report the solution structure of the Hox homeodomain in complex with the cell-cycle regulator, Geminin, which inhibits Hox transcriptional activity and enrolls Hox in cell proliferative control. Side-chain carboxylates of glutamates and aspartates in the C terminus of Geminin generate an overall charge pattern resembling the DNA phosphate backbone. These residues provide electrostatic interactions with homeodomain, which combine with the van der Waals contacts to form the stereospecific complex. We further showed that the interaction with Geminin is homeodomain subclass-selective and Hox paralog-specific, which relies on the stapling role of residues R43 and M54 in helix III and the basic amino acid cluster in the N terminus. Interestingly, we found that the C-terminal residue Ser184 of Geminin could be phosphorylated by Casein kinase II, resulting in the enhanced binding to Hox and more potent inhibitory effect on Hox transcriptional activity, indicating an additional layer of regulation. This structure provides insight into the molecular mechanism underlying homeodomain-protein recognition and may serve as a paradigm for interactions between homeodomains and DNA-competitive peptide inhibitors.

Structural basis for homeodomain recognition by the cell-cycle regulator Geminin.,Zhou B, Liu C, Xu Z, Zhu G Proc Natl Acad Sci U S A. 2012 May 21. PMID:22615398<ref>PMID:22615398</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2lp0" style="background-color:#fffaf0;"></div>

==See Also==
*[[Hox protein|Hox protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Liu C]]
[[Category: Xu Z]]
[[Category: Zhou B]]
[[Category: Zhu G]]