View source for 2lvj


==solution structure of hemi-Mg-bound Phl p 7==
<StructureSection load='2lvj' size='340' side='right'caption='[[2lvj]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lvj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phleum_pratense Phleum pratense]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LVJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvj OCA], [https://pdbe.org/2lvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lvj RCSB], [https://www.ebi.ac.uk/pdbsum/2lvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/POLC7_PHLPR POLC7_PHLPR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Polcalcins are small EF-hand proteins believed to assist in regulating pollen-tube growth. Phl p 7, from timothy grass (Phleum pratense), crystallizes as a domain-swapped dimer at low pH. This study describes the solution structures of the recombinant protein in buffered saline at pH 6.0, containing either 5.0 mM EDTA, 5.0 mM Mg(2+) , or 100 muM Ca(2+) . Phl p 7 is monomeric in all three ligation states. In the apo-form, both EF-hand motifs reside in the closed conformation, with roughly antiparallel N- and C-terminal helical segments. In 5.0 mM Mg(2+) , the divalent ion is bound by EF-hand 2, perturbing interhelical angles and imposing more regular helical structure. The structure of Ca(2+) -bound Phl p 7 resembles that previously reported for Bet v 4-likewise exposing apolar surface to the solvent. Occluded in the apo- and Mg(2+) -bound forms, this surface presumably provides the docking site for Phl p 7 targets. Unlike Bet v 4, EF-hand 2 in Phl p 7 includes five potential anionic ligands, due to replacement of the consensus serine residue at -x (residue 55 in Phl p 7) with aspartate. In the Phl p 7 crystal structure, D55 functions as a helix cap for helix D. In solution, however, D55 apparently serves as a ligand to the bound Ca(2+) . When Mg(2+) resides in site 2, the D55 carboxylate withdraws to a distance consistent with a role as an outer-sphere ligand. (15) N relaxation data, collected at 600 MHz, indicate that backbone mobility is limited in all three ligation states. Proteins 2013. (c) 2012 Wiley Periodicals, Inc.

Solution structures of polcalcin Phl p 7 in three ligation states: Apo-, hemi-Mg(2+) -bound, and fully Ca(2+) -bound.,Henzl MT, Sirianni AG, Wycoff WG, Tan A, Tanner JJ Proteins. 2013 Feb;81(2):300-15. doi: 10.1002/prot.24186. Epub 2012 Nov 12. PMID:23011803<ref>PMID:23011803</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2lvj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Phleum pratense]]
[[Category: Henzl MT]]
[[Category: Tanner JJ]]