View source for 2lxv


==Dimeric pil-e g-quadruplex dna from neisseria gonorrhoeae, NMR 11 structures==
<StructureSection load='2lxv' size='340' side='right'caption='[[2lxv]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lxv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LXV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lxv OCA], [https://pdbe.org/2lxv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lxv RCSB], [https://www.ebi.ac.uk/pdbsum/2lxv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lxv ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Neisseria gonorrhoeae is an obligate human pathogen that can escape immune surveillance through antigenic variation of surface structures such as pili. A G-quadruplex-forming (G4) sequence (5'-G(3)TG(3)TTG(3)TG(3)) located upstream of the N. gonorrhoeae pilin expression locus (pilE) is necessary for initiation of pilin antigenic variation, a recombination-based, high-frequency, diversity-generation system. We have determined NMR-based structures of the all parallel-stranded monomeric and 5' end-stacked dimeric pilE G-quadruplexes in monovalent cation-containing solutions. We demonstrate that the three-layered all parallel-stranded monomeric pilE G-quadruplex containing single-residue double-chain reversal loops, which can be modeled without steric clashes into the 3 nt DNA-binding site of RecA, binds and promotes E. coli RecA-mediated strand exchange in vitro. We discuss how interactions between RecA and monomeric pilE G-quadruplex could facilitate the specialized recombination reactions leading to pilin diversification.

RecA-Binding pilE G4 Sequence Essential for Pilin Antigenic Variation Forms Monomeric and 5' End-Stacked Dimeric Parallel G-Quadruplexes.,Kuryavyi V, Cahoon LA, Seifert HS, Patel DJ Structure. 2012 Oct 16. pii: S0969-2126(12)00347-4. doi:, 10.1016/j.str.2012.09.013. PMID:23085077<ref>PMID:23085077</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Kuryavyi V]]
[[Category: Patel DJ]]