View source for 2lzo


==Spatial structure of Pi-AnmTX Ugr 9a-1==
<StructureSection load='2lzo' size='340' side='right'caption='[[2lzo]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lzo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Urticina Urticina]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LZO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lzo OCA], [https://pdbe.org/2lzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lzo RCSB], [https://www.ebi.ac.uk/pdbsum/2lzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lzo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TX9A_URTGR TX9A_URTGR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Three novel peptides were isolated from the venom of the sea anemone Urticina grebelnyi. All of them are 29 amino acid peptides cross-linked by two disulfide bridges, with a primary structure similar to other sea anemone peptides belonging to structural group 9a. The structure of the gene encoding the shared precursor protein of the identified peptides was determined. One peptide, pi-AnmTX Ugr 9a-1 (short name Ugr 9-1), produced a reversible inhibition effect on both the transient and the sustained current of human ASIC3 channels expressed in Xenopus laevis oocytes. It completely blocked the transient component (IC50 10 +/- 0.6 mum) and partially (48 +/- 2%) inhibited the amplitude of the sustained component (IC50 1.44 +/- 0.19 mum). Using in vivo tests in mice, Ugr 9-1 significantly reversed inflammatory and acid-induced pain. The other two novel peptides, AnmTX Ugr 9a-2 (Ugr 9-2) and AnmTX Ugr 9a-3 (Ugr 9-3), did not inhibit the ASIC3 current. NMR spectroscopy revealed that Ugr 9-1 has an uncommon spatial structure, stabilized by two S-S bridges, with three classical beta-turns and twisted beta-hairpin without interstrand disulfide bonds. This is a novel peptide spatial structure that we propose to name boundless beta-hairpin.

Sea Anemone Peptide with Uncommon beta-Hairpin Structure Inhibits Acid-sensing Ion Channel 3 (ASIC3) and Reveals Analgesic Activity.,Osmakov DI, Kozlov SA, Andreev YA, Koshelev SG, Sanamyan NP, Sanamyan KE, Dyachenko IA, Bondarenko DA, Murashev AN, Mineev KS, Arseniev AS, Grishin EV J Biol Chem. 2013 Aug 9;288(32):23116-27. doi: 10.1074/jbc.M113.485516. Epub 2013, Jun 25. PMID:23801332<ref>PMID:23801332</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2lzo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Urticina]]
[[Category: Arseniev A]]
[[Category: Mineev K]]