View source for 2m6e


==NMR solution structure of cis (minor) form of In936 in Methanol==
<StructureSection load='2m6e' size='340' side='right'caption='[[2m6e]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2m6e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_inscriptus Conus inscriptus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M6E FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DLE:D-LEUCINE'>DLE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m6e OCA], [https://pdbe.org/2m6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m6e RCSB], [https://www.ebi.ac.uk/pdbsum/2m6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m6e ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Conformational diversity or "shapeshifting" in cyclic peptide natural products can, in principle, confer a single molecular entity with the property of binding to multiple receptors. Conformational equilibria have been probed in the contryphans, which are peptides derived from Conus venom possessing a 23-membered cyclic disulfide moiety. The natural sequences derived from Conus inscriptus, GCV(D) LYPWC* (In936) and Conus loroisii, GCP(D) WDPWC* (Lo959) differ in the number of proline residues within the macrocyclic ring. Structural characterisation of distinct conformational states arising from cis-trans equilibria about Xxx-Pro bonds is reported. Isomerisation about the C2-P3 bond is observed in the case of Lo959 and about the Y5-P6 bond in In936. Evidence is presented for as many as four distinct species in the case of the synthetic analogue V3P In936. The Tyr-Pro-Trp segment in In936 is characterised by distinct sidechain orientations as a consequence of aromatic/proline interactions as evidenced by specific sidechain-sidechain nuclear Overhauser effects and ring current shifted proton chemical shifts. Molecular dynamics simulations suggest that Tyr5 and Trp7 sidechain conformations are correlated and depend on the geometry of the Xxx-Pro bond. Thermodynamic parameters are derived for the cis&lt;--&gt; trans equilibrium for In936. Studies on synthetic analogues provide insights into the role of sequence effects in modulating isomerisation about Xxx-Pro bonds.

Conformational Diversity in Contryphans from Conus Venom: cis-trans Isomerisation and Aromatic/Proline Interactions in the 23-Membered Ring of a 7-Residue Peptide Disulfide Loop.,Sonti R, Gowd KH, Rao KN, Ragothama S, Rodriguez A, Perez JJ, Balaram P Chemistry. 2013 Nov 4;19(45):15175-89. doi: 10.1002/chem.201301722. Epub 2013 Sep, 23. PMID:24115170<ref>PMID:24115170</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2m6e" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Conus inscriptus]]
[[Category: Large Structures]]
[[Category: Sonti R]]