View source for 2mao


==NMR structure of region 2 of E. coli sigmaE==
<StructureSection load='2mao' size='340' side='right'caption='[[2mao]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mao]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH1 Escherichia coli DH1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MAO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mao OCA], [https://pdbe.org/2mao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mao RCSB], [https://www.ebi.ac.uk/pdbsum/2mao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mao ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli sigmaE, which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors.

Structural basis for -10 promoter element melting by environmentally induced sigma factors.,Campagne S, Marsh ME, Capitani G, Vorholt JA, Allain FH Nat Struct Mol Biol. 2014 Feb 16. doi: 10.1038/nsmb.2777. PMID:24531660<ref>PMID:24531660</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2mao" style="background-color:#fffaf0;"></div>

==See Also==
*[[Sigma factor 3D structures|Sigma factor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli DH1]]
[[Category: Large Structures]]
[[Category: Allain FH-T]]
[[Category: Campagne S]]
[[Category: Vorholt JA]]