View source for 2mcz


==CR1 Sushi domains 1 and 2==
<StructureSection load='2mcz' size='340' side='right'caption='[[2mcz]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mcz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MCZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MCZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mcz OCA], [https://pdbe.org/2mcz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mcz RCSB], [https://www.ebi.ac.uk/pdbsum/2mcz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mcz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CR1_HUMAN CR1_HUMAN] Mediates cellular binding of particles and immune complexes that have activated complement.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To survive and replicate within the human host, malaria parasites must invade erythrocytes. Invasion can be mediated by the P. falciparum reticulocyte-binding homologue protein 4 (PfRh4) on the merozoite surface interacting with complement receptor type one (CR1, CD35) on the erythrocyte membrane. The PfRh4 attachment site lies within the three N-terminal complement control protein modules (CCPs 1-3) of CR1, which intriguingly also accommodate binding and regulatory sites for the key complement activation-specific proteolytic products, C3b and C4b. One of these regulatory activities is decay-accelerating activity. While PfRh4 does not impact C3b/C4b binding, it does inhibit this convertase disassociating capability. Here, we have employed ELISA, co-immunoprecipitation and surface plasmon resonance to demonstrate that CCP 1 contains all the critical residues for PfRh4 interaction. We fine mapped by homologous substitution mutagenesis the PfRh4-binding site on CCP 1 and visualized it with a solution structure of CCPs 1-3 derived by NMR and SAXS. We cross-validated these results by creating an artificial PfRh4-binding site through substitution of putative PfRh4-interacting residues from CCP 1 into their homologous positions within CCP 8; strikingly, this engineered binding site had an approximately 30-fold higher affinity for PfRh4 than the native one in CCP 1. These experiments define a candidate site on CR1 by which P. falciparum merozoites gain access to human erythrocytes in a non-sialic acid dependent pathway of merozoite invasion.

Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor.,Park HJ, Guariento M, Maciejewski M, Hauhart R, Tham WH, Cowman AF, Schmidt CQ, Mertens HD, Liszewski MK, Hourcade DE, Barlow PN, Atkinson JP J Biol Chem. 2013 Nov 8. PMID:24214979<ref>PMID:24214979</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mcz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Atkinson JP]]
[[Category: Barlow PN]]
[[Category: Cowman AF]]
[[Category: Guariento MJ]]
[[Category: Hauart R]]
[[Category: Hourcade D]]
[[Category: Liszewski KM]]
[[Category: Maciejewski M]]
[[Category: Martens H]]
[[Category: Park HJ]]
[[Category: Schmidt CQ]]
[[Category: Tham W]]