View source for 2mhk


==E. coli LpoA N-terminal domain==
<StructureSection load='2mhk' size='340' side='right'caption='[[2mhk]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mhk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MHK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mhk OCA], [https://pdbe.org/2mhk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mhk RCSB], [https://www.ebi.ac.uk/pdbsum/2mhk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mhk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LPOA_ECOLI LPOA_ECOLI] Regulator of peptidoglycan synthesis that is essential for the function of penicillin-binding protein 1A (PBP1a). Stimulates transpeptidase activity of PBP1a in vitro.<ref>PMID:21183073</ref> <ref>PMID:21183074</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The bacterial cell envelope contains the stress-bearing peptidoglycan layer, which is enlarged during cell growth and division by membrane-anchored synthases guided by cytoskeletal elements. In Escherichia coli, the major peptidoglycan synthase PBP1A requires stimulation by the outer-membrane-anchored lipoprotein LpoA. Whereas the C-terminal domain of LpoA interacts with PBP1A to stimulate its peptide crosslinking activity, little is known about the role of the N-terminal domain. Herein we report its NMR structure, which adopts an all-alpha-helical fold comprising a series of helix-turn-helix tetratricopeptide-repeat (TPR)-like motifs. NMR spectroscopy of full-length LpoA revealed two extended flexible regions in the C-terminal domain and limited, if any, flexibility between the N- and C-terminal domains. Analytical ultracentrifugation and small-angle X-ray scattering results are consistent with LpoA adopting an elongated shape, with dimensions sufficient to span from the outer membrane through the periplasm to interact with the peptidoglycan synthase PBP1A.

Elongated Structure of the Outer-Membrane Activator of Peptidoglycan Synthesis LpoA: Implications for PBP1A Stimulation.,Jean NL, Bougault CM, Lodge A, Derouaux A, Callens G, Egan AJ, Ayala I, Lewis RJ, Vollmer W, Simorre JP Structure. 2014 Jul 8;22(7):1047-54. doi: 10.1016/j.str.2014.04.017. Epub 2014, Jun 19. PMID:24954617<ref>PMID:24954617</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mhk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Bougault C]]
[[Category: Callens G]]
[[Category: Derouaux A]]
[[Category: Egan A]]
[[Category: Jean NL]]
[[Category: Lewis RJ]]
[[Category: Lodge A]]
[[Category: Simorre J]]
[[Category: Vollmer W]]