View source for 2mkd


==Human JAZ ZF3 Residues 168-227==
<StructureSection load='2mkd' size='340' side='right'caption='[[2mkd]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mkd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MKD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mkd OCA], [https://pdbe.org/2mkd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mkd RCSB], [https://www.ebi.ac.uk/pdbsum/2mkd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mkd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ZN346_HUMAN ZN346_HUMAN] Binds to dsRNA and DNA (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The interactions of the human double-stranded RNA-binding zinc finger protein JAZ with RNA or DNA were investigated using electrophoretic mobility-shift assays, isothermal calorimetry, and nuclear magnetic resonance spectroscopy. Consistent with previous reports, JAZ has very low affinity for duplex DNA or single-stranded RNA, but it binds preferentially to double-stranded RNA (dsRNA) with no detectable sequence specificity. The affinity of JAZ for dsRNA is unaffected by local structural features such as loops, overhangs, and bulges, provided a sufficient length of reasonably well-structured A-form RNA (about 18 bp for a single zinc finger) is present. Full-length JAZ contains four Cys2His2 zinc fingers (ZF1-4) and has the highest apparent affinity for dsRNA; two-finger constructs ZF12 and ZF23 have lower affinity, and ZF34 binds even more weakly. The fourth zinc finger, ZF4, has no measurable RNA-binding affinity. Single zinc finger constructs ZF1, ZF2, and ZF3 show evidence for multiple-site binding on the minimal RNA. Fitting of quantitative NMR titration and isothermal calorimetry data to a two-site binding model gave Kd1 approximately 10 muM and Kd2 approximately 100 muM. Models of JAZ-RNA complexes were generated using the high-ambiguity-driven biomolecular docking (HADDOCK) program. Single zinc fingers bind to the RNA backbone without sequence specificity, forming complexes with contacts between the RNA minor groove and residues in the N-terminal beta strands and between the major groove and residues in the helix-kink-helix motif. We propose that the non-sequence-specific interaction between the zinc fingers of JAZ with dsRNA is dependent only on the overall shape of the A-form RNA.

Structural Characterization of Interactions between the Double-Stranded RNA-Binding Zinc Finger Protein JAZ and Nucleic Acids.,Burge RG, Martinez-Yamout MA, Dyson HJ, Wright PE Biochemistry. 2014 Mar 11;53(9):1495-510. doi: 10.1021/bi401675h. Epub 2014 Feb, 27. PMID:24521053<ref>PMID:24521053</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mkd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Burge R]]
[[Category: Dyson J]]
[[Category: Martinez-Yamout M]]
[[Category: Wright P]]