View source for 2mr8


==holo structure of the Peptidyl Carrier Protein Domain 7 of the teicoplanin producing Non-ribosomal peptide synthetase==
<StructureSection load='2mr8' size='340' side='right'caption='[[2mr8]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mr8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinoplanes_teichomyceticus Actinoplanes teichomyceticus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MR8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mr8 OCA], [https://pdbe.org/2mr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mr8 RCSB], [https://www.ebi.ac.uk/pdbsum/2mr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mr8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q70AZ6_ACTTI Q70AZ6_ACTTI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The biosynthesis of the glycopeptide antibiotics, of which teicoplanin and vancomycin are representative members, relies on the combination of non-ribosomal peptide synthesis and modification of the peptide by cytochrome P450 (Oxy) enzymes while the peptide remains bound to the peptide synthesis machinery. We have structurally characterized the final peptidyl carrier protein domain of the teicoplanin non-ribosomal peptide synthetase machinery: this domain is believed to mediate the interactions with tailoring Oxy enzymes in addition to its function as a shuttle for intermediates between multiple non-ribosomal peptide synthetase domains. Using solution state NMR, we have determined structures of this PCP domain in two states, the apo and the post-translationally modified holo state, both of which conform to a four-helix bundle assembly. The structures exhibit the same general fold as the majority of known carrier protein structures, in spite of the complex biosynthetic role that PCP domains from the final non-ribosomal peptide synthetase module must play in glycopeptide antibiotic biosynthesis. These structures thus support the hypothesis that it is subtle rearrangements, rather than dramatic conformational changes, which govern carrier protein interactions and selectivity during non-ribosomal peptide synthesis. This article is protected by copyright. All rights reserved.

Structure of the terminal PCP domain of the non-ribosomal peptide synthetase in teicoplanin biosynthesis.,Haslinger K, Redfield C, Cryle MJ Proteins. 2015 Jan 13. doi: 10.1002/prot.24758. PMID:25586301<ref>PMID:25586301</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mr8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Actinoplanes teichomyceticus]]
[[Category: Large Structures]]
[[Category: Cryle MJ]]
[[Category: Haslinger K]]
[[Category: Maximowitsch E]]
[[Category: Redfield C]]