View source for 2muf


==Binding activity, structure, and immunogenicity of synthetic peptides derived from Plasmodium falciparum CelTOS and TRSP proteins==
<StructureSection load='2muf' size='340' side='right'caption='[[2muf]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2muf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MUF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2muf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2muf OCA], [https://pdbe.org/2muf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2muf RCSB], [https://www.ebi.ac.uk/pdbsum/2muf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2muf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/M1EUE6_PLAFA M1EUE6_PLAFA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Several sporozoite proteins have been associated with Plasmodium falciparum cell traversal and hepatocyte invasion, including the cell-traversal protein for ookinetes and sporozoites (CelTOS), and thrombospondin-related sporozoite protein (TRSP). CelTOS and TRSP amino acid sequences have been finely mapped to identify regions specifically binding to HeLa and HepG2 cells, respectively. Three high-activity binding peptides (HABPs) were found in CelTOS and one HABP was found in TRSP, all of them having high alpha-helical structure content. These HABPs' specific binding was sensitive to HeLa and HepG2 cells' pre-treatment with heparinase I and chondroitinase ABC. Despite their similarity at three-dimensional (3D) structural level, TRSP and TRAP HABPs located in the TSR domain did not compete for the same binding sites. CelTOS and TRSP HABPs were used as a template for designing modified sequences to then be assessed in the Aotus monkey experimental model. Antibodies directed against these modified HABPs were able to recognize both the native parasite protein by immunofluorescence assay and the recombinant protein (expressed in Escherichia coli) by Western blot and ELISA assays. The results suggested that these modified HABPs could be promising targets in designing a fully effective, antimalarial vaccine.

Binding activity, structure, and immunogenicity of synthetic peptides derived from Plasmodium falciparum CelTOS and TRSP proteins.,Curtidor H, Arevalo-Pinzon G, Bermudez A, Calderon D, Vanegas M, Patino LC, Patarroyo MA, Patarroyo ME Amino Acids. 2012 Jul;43(1):365-78. doi: 10.1007/s00726-011-1087-8. Epub 2011 Sep, 28. PMID:21952731<ref>PMID:21952731</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2muf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Plasmodium falciparum]]
[[Category: Arevalo-Pinzon G]]
[[Category: Bermudez A]]
[[Category: Calderon D]]
[[Category: Curtidor H]]
[[Category: Patarroyo M]]
[[Category: Patino L]]
[[Category: Vanegas M]]