View source for 2n81


==Solution Structure of Lipid Transfer Protein From Pea Pisum Sativum==
<StructureSection load='2n81' size='340' side='right'caption='[[2n81]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2n81]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N81 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n81 OCA], [https://pdbe.org/2n81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n81 RCSB], [https://www.ebi.ac.uk/pdbsum/2n81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n81 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NLTP1_PEA NLTP1_PEA] [https://www.uniprot.org/uniprot/A0A182DV18_PEA A0A182DV18_PEA] 
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== Publication Abstract from PubMed ==
BACKGROUND: Plant lipid transfer proteins (LTPs) assemble a family of small (7-9 kDa) ubiquitous cationic proteins with an ability to bind and transport lipids as well as participate in various physiological processes including defense against phytopathogens. They also form one of the most clinically relevant classes of plant allergens. Nothing is known to date about correlation between lipid-binding and IgE-binding properties of LTPs. The garden pea Pisum sativum is widely consumed crop and important allergenic specie of the legume family. This work is aimed at isolation of a novel LTP from pea seeds and characterization of its structural, functional, and allergenic properties. RESULTS: Three novel lipid transfer proteins, designated as Ps-LTP1-3, were found in the garden pea Pisum sativum, their cDNA sequences were determined, and mRNA expression levels of all the three proteins were measured at different pea organs. Ps-LTP1 was isolated for the first time from the pea seeds, and its complete amino acid sequence was determined. The protein exhibits antifungal activity and is a membrane-active compound that causes a leakage from artificial liposomes. The protein binds various lipids including bioactive jasmonic acid. Spatial structure of the recombinant uniformly (13)C,(15)N-labelled Ps-LTP1 was solved by heteronuclear NMR spectroscopy. In solution the unliganded protein represents the mixture of two conformers (relative populations ~ 85:15) which are interconnected by exchange process with characteristic time ~ 100 ms. Hydrophobic residues of major conformer form a relatively large internal tunnel-like lipid-binding cavity (van der Waals volume comes up to ~1000 A(3)). The minor conformer probably corresponds to the protein with the partially collapsed internal cavity. CONCLUSIONS: For the first time conformational heterogeneity in solution was shown for an unliganded plant lipid transfer protein. Heat denaturation profile and simulated gastrointestinal digestion assay showed that Ps-LTP1 displayed a high thermal and digestive proteolytic resistance proper for food allergens. The reported structural and immunological findings seem to describe Ps-LTP1 as potential cross-reactive allergen in LTP-sensitized patients, mostly Pru p 3(+) ones. Similarly to allergenic LTPs the potential IgE-binding epitope of Ps-LTP1 is located near the proposed entrance into internal cavity and could be involved in lipid-binding.

A novel lipid transfer protein from the pea Pisum sativum: isolation, recombinant expression, solution structure, antifungal activity, lipid binding, and allergenic properties.,Bogdanov IV, Shenkarev ZO, Finkina EI, Melnikova DN, Rumynskiy EI, Arseniev AS, Ovchinnikova TV BMC Plant Biol. 2016 Apr 30;16(1):107. doi: 10.1186/s12870-016-0792-6. PMID:27137920<ref>PMID:27137920</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pisum sativum]]
[[Category: Arseniev AS]]
[[Category: Bogdanov IV]]
[[Category: Finkina EI]]
[[Category: Melnikova DN]]
[[Category: Ovchinnikova TV]]
[[Category: Paramonov AS]]
[[Category: Rumynskiy EI]]
[[Category: Shenkarev ZO]]