View source for 2nbg


==Structure of the Geobacillus stearothermophilus IF2 G3-subdomain==
<StructureSection load='2nbg' size='340' side='right'caption='[[2nbg]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2nbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NBG FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nbg OCA], [https://pdbe.org/2nbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nbg RCSB], [https://www.ebi.ac.uk/pdbsum/2nbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nbg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IF2_GEOSE IF2_GEOSE] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
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== Publication Abstract from PubMed ==
Bacterial translation initiation factor IF2 complexed with GTP binds to the 30S ribosomal subunit, promotes ribosomal binding of fMet-tRNA, and favors the joining of the small and large ribosomal subunits yielding a 70S initiation complex ready to enter the translation elongation phase. Within the IF2 molecule subdomain G3, which is believed to play an important role in the IF2-30S interaction, is positioned between the GTP-binding G2 and the fMet-tRNA binding C-terminal subdomains. In this study the solution structure of subdomain G3 of Geobacillus stearothermophilus IF2 has been elucidated. G3 forms a core structure consisting of two beta-sheets with each four anti-parallel strands, followed by a C-terminal alpha-helix. In line with its role as linker between G3 and subdomain C1, this helix has no well-defined orientation but is endowed with a dynamic nature. The structure of the G3 core is that of a typical OB-fold module, similar to that of the corresponding subdomain of Thermus thermophilus IF2, and to that of other known RNA-binding modules such as IF2-C2, IF1 and subdomains II of elongation factors EF-Tu and EF-G. Structural comparisons have resulted in a model that describes the interaction between IF2-G3 and the 30S ribosomal subunit.

A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit.,Dongre R, Folkers GE, Gualerzi CO, Boelens R, Wienk H Protein Sci. 2016 Jul 1. doi: 10.1002/pro.2977. PMID:27364543<ref>PMID:27364543</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Large Structures]]
[[Category: Boelens R]]
[[Category: Dongre R]]
[[Category: Folkers GE]]
[[Category: Gualerzi CO]]
[[Category: Wienk H]]