View source for 2oz6


==Crystal Structure of Virulence Factor Regulator from Pseudomonas aeruginosa in complex with cAMP==
<StructureSection load='2oz6' size='340' side='right'caption='[[2oz6]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2oz6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OZ6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oz6 OCA], [https://pdbe.org/2oz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oz6 RCSB], [https://www.ebi.ac.uk/pdbsum/2oz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oz6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VFR_PSEAE VFR_PSEAE] Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/2oz6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oz6 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Virulence Factor Regulator (Vfr) enhances Pseudomonas aeruginosa pathogenicity through its role as a global transcriptional regulator. The crystal structure of Vfr shows it is a winged-helix DNA-binding protein like its homologue cAMP Receptor Protein (CRP). In addition to an expected primary cAMP-binding site, a second ligand-binding site is nestled between the N-terminal domain and the C-terminal helix-turn-helix domain. Unlike CRP, Vfr is a symmetric dimer in the absence of DNA. Removal of seven disordered N-terminal residues of Vfr prevents growth of P. aeruginosa.

Crystal Structure of Pseudomonas aeruginosa Virulence Factor Regulator.,Cordes TJ, Worzalla GA, Ginster AM, Forest KT J Bacteriol. 2011 Jun 10. PMID:21665969<ref>PMID:21665969</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2oz6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Bright AR]]
[[Category: Cordes TJ]]
[[Category: Forest KT]]