View source for 2rlh


==NMR structure of the antimicrobial peptide RP-1 bound to DPC micelles==
<StructureSection load='2rlh' size='340' side='right'caption='[[2rlh]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2rlh]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RLH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rlh OCA], [https://pdbe.org/2rlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rlh RCSB], [https://www.ebi.ac.uk/pdbsum/2rlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rlh ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Topologically, platelet factor-4 kinocidins consist of distinct N-terminal extended, C-terminal helical, and interposing gamma-core structural domains. The C-terminal alpha-helices autonomously confer direct microbicidal activity, and the synthetic antimicrobial peptide RP-1 is modeled upon these domains. In this study, the structure of RP-1 was assessed using several complementary techniques. The high-resolution structure of RP-1 was determined by NMR in anionic sodium dodecyl sulfate (SDS) and zwitterionic dodecylphosphocholine (DPC) micelles, which approximate prokaryotic and eukaryotic membranes, respectively. NMR data indicate the peptide assumes an amphipathic alpha-helical backbone conformation in both micelle environments. However, small differences were observed in the side-chain orientations of lysine, tyrosine, and phenylalanine residues in SDS versus DPC environments. NMR experiments with a paramagnetic probe indicated differences in positioning of the peptide within the two micelle types. Molecular dynamics (MD) simulations of the peptide in both micelle types were also performed to add insight into the peptide/micelle interactions and to assess the validity of this technique to predict the structure of peptides in complex with micelles. MD independently predicted RP-1 to interact only peripherally with the DPC micelle, leaving its spherical shape intact. In contrast, RP-1 entered deeply into and significantly distorted the SDS micelle. Overall, the experimental and MD results support a preferential specificity of RP-1 for anionic membranes over zwitterionic membranes. This specificity likely derives from differences in RP-1 interaction with distinct lipid systems, including subtle differences in side chain orientations, rather than gross changes in RP-1 structure in the two lipid environments.

Antimicrobial peptide RP-1 structure and interactions with anionic versus zwitterionic micelles.,Bourbigot S, Dodd E, Horwood C, Cumby N, Fardy L, Welch WH, Ramjan Z, Sharma S, Waring AJ, Yeaman MR, Booth V Biopolymers. 2009 Jan;91(1):1-13. PMID:18712851<ref>PMID:18712851</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2rlh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Booth V]]
[[Category: Bourbigot S]]
[[Category: Dodd E]]
[[Category: Horwood C]]