View source for 2ru5


==Designed Armadillo Repeat Protein Fragment (MAII)==
<StructureSection load='2ru5' size='340' side='right'caption='[[2ru5]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ru5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RU5 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ru5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ru5 OCA], [https://pdbe.org/2ru5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ru5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ru5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ru5 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Repeat proteins are built of modules, each of which constitutes a structural motif. We have investigated whether fragments of a designed consensus armadillo repeat protein (ArmRP) recognize each other. We examined a split ArmRP consisting of an N-capping repeat (denoted Y), three internal repeats (M), and a C-capping repeat (A). We demonstrate that the C-terminal MA fragment adopts a fold similar to the corresponding part of the entire protein. In contrast, the N-terminal YM2 fragment constitutes a molten globule. The two fragments form a 1:1 YM2:MA complex with a nanomolar dissociation constant essentially identical to the crystal structure of the continuous YM3A protein. Molecular dynamics simulations show that the complex is structurally stable over a 1 mus timescale and reveal the importance of hydrophobic contacts across the interface. We propose that the existence of a stable complex recapitulates possible intermediates in the early evolution of these repeat proteins.

Spontaneous self-assembly of engineered armadillo repeat protein fragments into a folded structure.,Watson RP, Christen MT, Ewald C, Bumbak F, Reichen C, Mihajlovic M, Schmidt E, Guntert P, Caflisch A, Pluckthun A, Zerbe O Structure. 2014 Jul 8;22(7):985-95. doi: 10.1016/j.str.2014.05.002. Epub 2014 Jun, 12. PMID:24931467<ref>PMID:24931467</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2ru5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Christen MT]]
[[Category: Plueckthun A]]
[[Category: Watson RP]]
[[Category: Zerbe O]]