View source for 2rvf


==Solution NMR structure of Monosiga brevicollis CRK/CRKL homolog (crka1) SH2 domain==
<StructureSection load='2rvf' size='340' side='right'caption='[[2rvf]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2rvf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Monosiga_brevicollis Monosiga brevicollis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RVF FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rvf OCA], [https://pdbe.org/2rvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rvf RCSB], [https://www.ebi.ac.uk/pdbsum/2rvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rvf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A9UZF4_MONBE A9UZF4_MONBE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CRK and CRKL adapter proteins play essential roles in development and cancer through their SRC homology 2 and 3 (SH2 and SH3) domains. To gain insight into the origin of their shared functions, we have investigated their evolutionary history. We propose a term, crk/crkl ancestral (crka), for orthologs in invertebrates before the divergence of CRK and CRKL in the vertebrate ancestor. We have isolated two orthologs expressed in the choanoflagellate Monosiga brevicollis, a unicellular relative to the metazoans. Consistent with its highly-conserved three-dimensional structure, the SH2 domain of M. brevicollis crka1 can bind to the mammalian CRK/CRKL SH2 binding consensus phospho-YxxP, and to the SRC substrate/focal adhesion protein BCAR1 (p130CAS) in the presence of activated SRC. These results demonstrate an ancient origin of the CRK/CRKL SH2-target recognition specificity. Although BCAR1 orthologs exist only in metazoans as identified by an N-terminal SH3 domain, YxxP motifs, and a C-terminal FAT-like domain, some pre-metazoan transmembrane proteins include several YxxP repeats in their cytosolic region, suggesting that they are remotely related to the BCAR1 substrate domain. Since the tyrosine kinase SRC also has a pre-metazoan origin, co-option of BCAR1-related sequences may have rewired the crka-dependent network to mediate adhesion signals in the metazoan ancestor.

A pre-metazoan origin of the CRK gene family and co-opted signaling network.,Shigeno-Nakazawa Y, Kasai T, Ki S, Kostyanovskaya E, Pawlak J, Yamagishi J, Okimoto N, Taiji M, Okada M, Westbrook J, Satta Y, Kigawa T, Imamoto A Sci Rep. 2016 Sep 30;6:34349. doi: 10.1038/srep34349. PMID:27686861<ref>PMID:27686861</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2rvf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Monosiga brevicollis]]
[[Category: Imamoto A]]
[[Category: Kasai T]]
[[Category: Kigawa T]]
[[Category: Pawlak J]]