View source for 2tbd


==SV40 T ANTIGEN DNA-BINDING DOMAIN, NMR, 30 STRUCTURES==
<StructureSection load='2tbd' size='340' side='right'caption='[[2tbd]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2tbd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2TBD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2tbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2tbd OCA], [https://pdbe.org/2tbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2tbd RCSB], [https://www.ebi.ac.uk/pdbsum/2tbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2tbd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q98ZP7_SV40 Q98ZP7_SV40] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tb/2tbd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2tbd ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel beta-sheet flanked by two alpha-helices on one side and one alpha-helix and one 3(10)-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (approximately 152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA.

Solution structure of the origin DNA-binding domain of SV40 T-antigen.,Luo X, Sanford DG, Bullock PA, Bachovchin WW Nat Struct Biol. 1996 Dec;3(12):1034-9. PMID:8946857<ref>PMID:8946857</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2tbd" style="background-color:#fffaf0;"></div>

==See Also==
*[[Large T Antigen|Large T Antigen]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Macaca mulatta polyomavirus 1]]
[[Category: Bachovchin WW]]
[[Category: Bullock PA]]
[[Category: Luo X]]
[[Category: Sanford DG]]