View source for 2uwj


==Structure of the heterotrimeric complex which regulates type III secretion needle formation==
<StructureSection load='2uwj' size='340' side='right'caption='[[2uwj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2uwj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_CHA Pseudomonas aeruginosa CHA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UWJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwj OCA], [https://pdbe.org/2uwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uwj RCSB], [https://www.ebi.ac.uk/pdbsum/2uwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PSCF_PSEAE PSCF_PSEAE] Major component of the type III secretion needle structure. Required for cytotoxicity on macrophages.<ref>PMID:16239085</ref> <ref>PMID:16115870</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uw/2uwj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uwj ConSurf].
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== Publication Abstract from PubMed ==
Type III secretion systems (T3SS), found in several Gram-negative pathogens, are nanomachines involved in the transport of virulence effectors directly into the cytoplasm of target cells. T3SS are essentially composed of basal membrane-embedded ring-like structures and a hollow needle formed by a single polymerized protein. Within the bacterial cytoplasm, the T3SS needle protein requires two distinct chaperones for stabilization before its secretion, without which the entire T3SS is nonfunctional. The 2.0-A x-ray crystal structure of the PscE-PscF(55-85)-PscG heterotrimeric complex from Pseudomonas aeruginosa reveals that the C terminus of the needle protein PscF is engulfed within the hydrophobic groove of the tetratricopeptide-like molecule PscG, indicating that the macromolecular scaffold necessary to stabilize the T3SS needle is totally distinct from chaperoned complexes between pilus- or flagellum-forming molecules. Disruption of specific PscG-PscF interactions leads to impairment of bacterial cytotoxicity toward macrophages, indicating that this essential heterotrimer, which possesses homologs in a wide variety of pathogens, is a unique attractive target for the development of novel antibacterials.

Structure of the heterotrimeric complex that regulates type III secretion needle formation.,Quinaud M, Ple S, Job V, Contreras-Martel C, Simorre JP, Attree I, Dessen A Proc Natl Acad Sci U S A. 2007 May 8;104(19):7803-8. Epub 2007 Apr 30. PMID:17470796<ref>PMID:17470796</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2uwj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa CHA]]
[[Category: Attree I]]
[[Category: Contreras-Martel C]]
[[Category: Dessen A]]
[[Category: Job V]]
[[Category: Ple S]]
[[Category: Quinaud M]]
[[Category: Simorre JP]]