View source for 2wnd


==Structure of an S100A7 triple mutant==
<StructureSection load='2wnd' size='340' side='right'caption='[[2wnd]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2wnd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WND OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WND FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wnd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wnd OCA], [https://pdbe.org/2wnd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wnd RCSB], [https://www.ebi.ac.uk/pdbsum/2wnd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wnd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/S10A7_HUMAN S10A7_HUMAN] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/2wnd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wnd ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
S100A7 (psoriasin) is a calcium and zinc-binding protein implicated in breast cancer. We have shown previously that S100A7 enhances survival mechanisms in breast cells through an interaction with c-jun activation domain binding protein 1 (Jab1), and an engineered S100A7 triple mutant (Asp(56)Gly, Leu(78)Met, and Gln(88)Lys - S100A7(3)) ablates Jab1 binding. Here we extend these results to include defined breast cancer cell lines and demonstrate a disrupted S100A7(3)/Jab1 phenotype is maintained. To establish the basis for the abrogated Jab1 binding we have recombinantly produced S100A7(3), demonstrated that it retains the ability to form an exceptionally thermostable dimer and solved the three dimensional crystal structure to 1.6 A. Despite being positioned at the dimer interface, the Leu(78)Met mutation is easily accommodated and contributes to a methionine rich pocket formed by Met(12), Met(15), Met(34). In addition to altering the surface charge, the Gln(88)Lys mutation results in a nearby rotameric shift in Tyr(85) leading to a substantially reorganized surface cavity and may influence zinc binding. The final mutation of Asp(56) to Gly results in the largest structural perturbation shortening helix IV by one full turn. It is noteworthy that position 56 lies in one of two divergent clusters between S100A7 and the functionally distinct yet highly homologous S100A15. The structure of S100A7(3) provides a unique perspective from which to characterize the S100A7-Jab1 interaction and better understand the distinct functions between S100A7 and its closely related paralog S100A15. (c) 2009 The Protein Society.

Structural and functional characterization of a triple mutant form of S100A7 defective for Jab1 binding.,West NR, Farnell B, Murray JI, Hof F, Watson PH, Boulanger MJ Protein Sci. 2009 Oct 20. PMID:19844956<ref>PMID:19844956</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2wnd" style="background-color:#fffaf0;"></div>

==See Also==
*[[S100 proteins 3D structures|S100 proteins 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Boulanger MJ]]
[[Category: Farnell B]]
[[Category: Watson PH]]
[[Category: West NR]]