View source for 2xbg


==Crystal Structure of YCF48 from Thermosynechococcus elongatus==
<StructureSection load='2xbg' size='340' side='right'caption='[[2xbg]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2xbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus Thermosynechococcus vestitus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XBG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xbg OCA], [https://pdbe.org/2xbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xbg RCSB], [https://www.ebi.ac.uk/pdbsum/2xbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xbg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YCF48_THEVB YCF48_THEVB] A factor required for optimal assembly of photosystem II (PSII), acting in the early stages of PSII assembly. Also plays a role in replacement of photodamaged D1 (psbA). Assists YidC in synthesis of chlorophyll-binding proteins.[HAMAP-Rule:MF_01348]<ref>PMID:30061392</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Robust photosynthesis in chloroplasts and cyanobacteria requires the participation of accessory proteins to facilitate the assembly and maintenance of the photosynthetic apparatus located within the thylakoid membranes. The highly conserved Ycf48 protein acts early in the biogenesis of the oxygen-evolving photosystem II (PSII) complex by binding to newly synthesized precursor D1 subunit and by promoting efficient association with the D2 protein to form a PSII reaction center (PSII RC) assembly intermediate. Ycf48 is also required for efficient replacement of damaged D1 during the repair of PSII. However, the structural features underpinning Ycf48 function remain unclear. Here we show that Ycf48 proteins encoded by the thermophilic cyanobacterium Thermosynechococcus elongatus and the red alga Cyanidioschyzon merolae form seven-bladed beta-propellers with the 19-aa insertion characteristic of eukaryotic Ycf48 located at the junction of blades 3 and 4. Knowledge of these structures has allowed us to identify a conserved "Arg patch" on the surface of Ycf48 that is important for binding of Ycf48 to PSII RCs but also to larger complexes, including trimeric photosystem I (PSI). Reduced accumulation of chlorophyll in the absence of Ycf48 and the association of Ycf48 with PSI provide evidence of a more wide-ranging role for Ycf48 in the biogenesis of the photosynthetic apparatus than previously thought. Copurification of Ycf48 with the cyanobacterial YidC protein insertase supports the involvement of Ycf48 during the cotranslational insertion of chlorophyll-binding apopolypeptides into the membrane.

Ycf48 involved in the biogenesis of the oxygen-evolving photosystem II complex is a seven-bladed beta-propeller protein.,Yu J, Knoppova J, Michoux F, Bialek W, Cota E, Shukla MK, Straskova A, Pascual Aznar G, Sobotka R, Komenda J, Murray JW, Nixon PJ Proc Natl Acad Sci U S A. 2018 Aug 14;115(33):E7824-E7833. doi:, 10.1073/pnas.1800609115. Epub 2018 Jul 30. PMID:30061392<ref>PMID:30061392</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2xbg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermosynechococcus vestitus]]
[[Category: Michoux F]]
[[Category: Murray JW]]
[[Category: Nixon P]]
[[Category: Takasaka K]]