View source for 2xkx


==Single particle analysis of PSD-95 in negative stain==
<SX load='2xkx' size='340' side='right' viewer='molstar' caption='[[2xkx]], [[Resolution|resolution]] 22.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2xkx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XKX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy , Hybrid , X-ray solution scattering, [[Resolution|Resolution]] 22.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkx OCA], [https://pdbe.org/2xkx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xkx RCSB], [https://www.ebi.ac.uk/pdbsum/2xkx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xkx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DLG4_RAT DLG4_RAT] Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.<ref>PMID:15317815</ref> <ref>PMID:15358863</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using Transmission Electron Microscopy in negative stain. Three types of complexes were observed in electron micrographs corresponding to a 1:1 complex, a large self-enclosed tetrad complex and extended chains of linked channel domains. Using models derived from small angle X-ray scattering experiments in which high resolution structures from X-ray crystallographic and Nuclear Magnetic Resonance studies are positioned, the envelopes from single particle analysis can be resolved as a Kir2.1NC(4):PSD-95 complex and a tetrad of this unit (Kir2.1NC(4):PSD-95)(4). The tetrad complex shows the close association of the Kir2.1 cytoplasmic domains and the influence of PSD-95 mediated self-assembly on the clustering of these channels.

Self-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95.,Fomina S, Howard TD, Sleator OK, Golovanova M, O'Ryan L, Leyland ML, Grossmann JG, Collins RF, Prince SM Biochim Biophys Acta. 2011 Jul 5;1808(10):2374-2389. PMID:21756874<ref>PMID:21756874</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2xkx" style="background-color:#fffaf0;"></div>

==See Also==
*[[Postsynaptic density protein|Postsynaptic density protein]]
*[[Postsynaptic density protein 3D structures|Postsynaptic density protein 3D structures]]
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Collins RF]]
[[Category: Fomina S]]
[[Category: Golovanova M]]
[[Category: Grossmann JG]]
[[Category: Howard TD]]
[[Category: Leyland ML]]
[[Category: O'Ryan L]]
[[Category: Prince SM]]
[[Category: Sleator OK]]