View source for 2xlc


==Acetyl xylan esterase from Bacillus pumilus CECT5072 bound to paraoxon==
<StructureSection load='2xlc' size='340' side='right'caption='[[2xlc]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2xlc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_pumilus Bacillus pumilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XLC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlc OCA], [https://pdbe.org/2xlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xlc RCSB], [https://www.ebi.ac.uk/pdbsum/2xlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xlc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9K5F2_BACPU Q9K5F2_BACPU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Organophosphorus insecticides and nerve agents irreversibly inhibit serine hydrolase superfamily enzymes. One enzyme of this superfamily, the industrially important (for beta-lactam antibiotic synthesis) AXE/CAH (acetyl xylan esterase/cephalosporin acetyl hydrolase) from the biotechnologically valuable organism Bacillus pumilus, exhibits low sensitivity to the organophosphate paraoxon (diethyl-p-nitrophenyl phosphate, also called paraoxon-ethyl), reflected in a high Ki for it (~5 mM) and in a slow formation (t(1/2)~1 min) of the covalent adduct of the enzyme and for DEP (E-DEP, enzyme-diethyl phosphate, i.e. enzyme-paraoxon). The crystal structure of the E-DEP complex determined at 2.7 A resolution (1 A=0.1 nm) reveals strain in the active Ser181-bound organophosphate as a likely cause for the limited paraoxon sensitivity. The strain results from active-site-size limitation imposed by bulky conserved aromatic residues that may exclude as substrates esters having acyl groups larger than acetate. Interestingly, in the doughnut-like homohexamer of the enzyme, the six active sites are confined within a central chamber formed between two 60 degrees -staggered trimers. The exclusive access to this chamber through a hole around the three-fold axis possibly limits the size of the xylan natural substrates. The enzyme provides a rigid scaffold for catalysis, as reflected in the lack of movement associated with paraoxon adduct formation, as revealed by comparing this adduct structure with that also determined in the present study at 1.9 A resolution for the paraoxon-free enzyme.

The crystal structure of the cephalosporin deacetylating enzyme acetyl xylan esterase bound to paraoxon explains the low sensitivity of this serine hydrolase to organophosphate inactivation.,Montoro-Garcia S, Gil-Ortiz F, Garcia-Carmona F, Polo LM, Rubio V, Sanchez-Ferrer A Biochem J. 2011 Jun 1;436(2):321-30. PMID:21382014<ref>PMID:21382014</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2xlc" style="background-color:#fffaf0;"></div>

==See Also==
*[[Acetylxylan esterase 3D structures|Acetylxylan esterase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus pumilus]]
[[Category: Large Structures]]
[[Category: Gil-Ortiz F]]
[[Category: Montoro-Garcia S]]
[[Category: Polo LM]]
[[Category: Rubio V]]
[[Category: Sanchez-Ferrer A]]