View source for 2xsc


==Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coli==
<StructureSection load='2xsc' size='340' side='right'caption='[[2xsc]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2xsc]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bov 1bov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XSC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.052&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xsc OCA], [https://pdbe.org/2xsc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xsc RCSB], [https://www.ebi.ac.uk/pdbsum/2xsc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xsc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/STXB_BPH19 STXB_BPH19] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Shiga toxin family, a group of cytotoxins associated with diarrhoeal diseases and the haemolytic uraemic syndrome, includes Shiga toxin from Shigella dysenteriae type 1 and verotoxins produced by enteropathogenic Escherichia coli. The family belongs to the A-B class of bacterial toxins, which includes the cholera toxin family, pertussis and diphtheria toxins. These toxins all have bipartite structures consisting of an enzymatic A subunit associated with a B oligomer which binds to specific cell-surface receptors, but their amino-acid sequences and pathogenic mechanisms differ. We have determined the crystal structure of the B oligomer of verotoxin-1 from E. coli. The structure unexpectedly resembles that of the B oligomer of the cholera toxin-like heat-labile enterotoxin from E. coli, despite the absence of detectable sequence similarity between these two proteins. This result implies a distant evolutionary relationship between the Shiga toxin and cholera toxin families. We suggest that the cell surface receptor-binding site lies in a cleft between adjacent subunits of the B pentamer, providing a potential target for drugs and vaccines to prevent toxin binding and effect.

Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coli.,Stein PE, Boodhoo A, Tyrrell GJ, Brunton JL, Read RJ Nature. 1992 Feb 20;355(6362):748-50. PMID:1741063<ref>PMID:1741063</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2xsc" style="background-color:#fffaf0;"></div>

==See Also==
*[[Shiga toxin|Shiga toxin]]
*[[Shiga toxin 3D structures|Shiga toxin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Boodhoo A]]
[[Category: Brunton JL]]
[[Category: Bunkoczi G]]
[[Category: Oeffner RD]]
[[Category: Read RJ]]
[[Category: Stein PE]]
[[Category: Tyrrell GJ]]