View source for 2xvk


==crystal structure of alpha-xylosidase (GH31) from Cellvibrio japonicus in complex with 5-fluoro-alpha-D-xylopyranosyl fluoride==
<StructureSection load='2xvk' size='340' side='right'caption='[[2xvk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2xvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XVK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.503&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FFX:(2R,3S,5R,6S)-2,6-DIFLUOROOXANE-3,4,5-TRIOL'>FFX</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xvk OCA], [https://pdbe.org/2xvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xvk RCSB], [https://www.ebi.ac.uk/pdbsum/2xvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xvk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B3PBD9_CELJU B3PBD9_CELJU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The desire for improved methods of biomass conversion to fuels and feedstocks has re-awakened interest in the enzymology of plant cell wall degradation. The complex polysaccharide xyloglucan is abundant in plant matter, where it may account for up to 20% of the total primary cell wall carbohydrates. Despite this, few studies have focussed on xyloglucan saccharification, which requires a consortium of enzymes including endo-xyloglucanases, alpha-xylosidases, betagalactosidases and alpha-l-fucosidases, among others. Here, we present the characterisation of Xyl31A, a key alpha-xylosidase in xyloglucan utilisation by the model Gram-negative soil saprophyte Cellvibrio japonicus. CjXyl31A exhibits high regiospecificity for the hydrolysis xylogluco-oligosaccharides, with a particular preference for longer substrates. Crystallographic structures of both the apo enzyme and the trapped covalent 5-fluoro-beta-xylosyl-enzyme intermediate, together with docking studies with the XXXG heptasaccharide, revealed, for the first time in glycoside hydrolase family 31, the importance of a PA14 domain insert in the recognition of longer oligosaccharides by extension of the active site pocket. The observation that CjXyl31A was localised to the outer membrane provided support for a biological model of xyloglucan utilisation by C. japonicus, in which xylogluco-oligosaccharides generated by the action of a secreted endo-xyloglucanase are ultimately degraded in close proximity to the cell surface. Moreover, this study diversifies the toolbox of glycosidases for the specific modification and saccharification of cell wall polymers for biotechnological applications.

Structural and enzymatic characterisation of a Glycoside Hydrolase Family 31 alphaxylosidase from Cellvibrio japonicus involved in xyloglucan saccharification.,Larsbrink J, Izumi A, Ibatullin F, Nakhai A, Gilbert HJ, Davies GJ, Brumer H Biochem J. 2011 Mar 22. PMID:21426303<ref>PMID:21426303</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2xvk" style="background-color:#fffaf0;"></div>

==See Also==
*[[Xylosidase 3D structures|Xylosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cellvibrio japonicus]]
[[Category: Large Structures]]
[[Category: Brumer H]]
[[Category: Davies GJ]]
[[Category: Gilbert HJ]]
[[Category: Ibatullin F]]
[[Category: Izumi A]]
[[Category: Larsbrink J]]
[[Category: Nakhai A]]