View source for 2yg2


==Structure of apolioprotein M in complex with Sphingosine 1-Phosphate==
<StructureSection load='2yg2' size='340' side='right'caption='[[2yg2]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2yg2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YG2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=S1P:(2S,3R,4E)-2-AMINO-3-HYDROXYOCTADEC-4-EN-1-YL+DIHYDROGEN+PHOSPHATE'>S1P</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yg2 OCA], [https://pdbe.org/2yg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yg2 RCSB], [https://www.ebi.ac.uk/pdbsum/2yg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yg2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/APOM_HUMAN APOM_HUMAN] Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.<ref>PMID:17525477</ref> <ref>PMID:19733574</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protection of the endothelium is provided by circulating sphingosine-1-phosphate (S1P), which maintains vascular integrity. We show that HDL-associated S1P is bound specifically to both human and murine apolipoprotein M (apoM). Thus, isolated human ApoM(+) HDL contained S1P, whereas ApoM(-) HDL did not. Moreover, HDL in Apom(-/-) mice contains no S1P, whereas HDL in transgenic mice overexpressing human apoM has an increased S1P content. The 1.7-A structure of the S1P-human apoM complex reveals that S1P interacts specifically with an amphiphilic pocket in the lipocalin fold of apoM. Human ApoM(+) HDL induced S1P(1) receptor internalization, downstream MAPK and Akt activation, endothelial cell migration, and formation of endothelial adherens junctions, whereas apoM(-) HDL did not. Importantly, lack of S1P in the HDL fraction of Apom(-/-) mice decreased basal endothelial barrier function in lung tissue. Our results demonstrate that apoM, by delivering S1P to the S1P(1) receptor on endothelial cells, is a vasculoprotective constituent of HDL.

Endothelium-protective sphingosine-1-phosphate provided by HDL-associated apolipoprotein M.,Christoffersen C, Obinata H, Kumaraswamy SB, Galvani S, Ahnstrom J, Sevvana M, Egerer-Sieber C, Muller YA, Hla T, Nielsen LB, Dahlback B Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9613-8. Epub 2011 May 23. PMID:21606363<ref>PMID:21606363</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2yg2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Ahnstrom J]]
[[Category: Christoffersen C]]
[[Category: Dahlback B]]
[[Category: Egerer-Sieber C]]
[[Category: Galvani S]]
[[Category: Gowda S]]
[[Category: Hla T]]
[[Category: Muller YA]]
[[Category: Nielsen L]]
[[Category: Obinata H]]
[[Category: Sevvana M]]