View source for 2ywf


==Crystal structure of GMPPNP-bound LepA from Aquifex aeolicus==
<StructureSection load='2ywf' size='340' side='right'caption='[[2ywf]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ywf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YWF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ywf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ywf OCA], [https://pdbe.org/2ywf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ywf RCSB], [https://www.ebi.ac.uk/pdbsum/2ywf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ywf ProSAT], [https://www.topsan.org/Proteins/RSGI/2ywf TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LEPA_AQUAE LEPA_AQUAE] Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yw/2ywf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ywf ConSurf].
<div style="clear:both"></div>

==See Also==
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
*[[Escherichia coli LepA%2C the ribosomal back translocase|Escherichia coli LepA%2C the ribosomal back translocase]]
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
__TOC__
</StructureSection>
[[Category: Aquifex aeolicus VF5]]
[[Category: Large Structures]]
[[Category: Hanawa-Suetsugu K]]
[[Category: Kaminishi T]]
[[Category: Kawazoe M]]
[[Category: Nakayama-Ushikoshi R]]
[[Category: Nishino A]]
[[Category: Shirouzu M]]
[[Category: Takemoto C]]
[[Category: Terada T]]
[[Category: Yokoyama S]]