View source for 2znm


==Oxidoreductase NmDsbA3 from Neisseria meningitidis==
<StructureSection load='2znm' size='340' side='right'caption='[[2znm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2znm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_B Neisseria meningitidis serogroup B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZNM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2znm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2znm OCA], [https://pdbe.org/2znm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2znm RCSB], [https://www.ebi.ac.uk/pdbsum/2znm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2znm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9K0Z4_NEIMB Q9K0Z4_NEIMB] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zn/2znm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2znm ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DsbA is an enzyme found in the periplasm of Gram-negative bacteria that catalyzes the formation of disulfide bonds in a diverse array of protein substrates, many of which are involved in bacterial pathogenesis. Although most bacteria possess only a single essential DsbA, Neisseria meningitidis is unusual in that it possesses three DsbAs, although the reason for this additional redundancy is unclear. Two of these N. meningitidis enzymes (NmDsbA1 and NmDsbA2) play an important role in meningococcal attachment to human epithelial cells, whereas NmDsbA3 is considered to have a narrow substrate repertoire. To begin to address the role of DsbAs in the pathogenesis of N. meningitidis, we have determined the structure of NmDsbA3 to 2.3-A resolution. Although the sequence identity between NmDsbA3 and other DsbAs is low, the NmDsbA3 structure adopted a DsbA-like fold. Consistent with this finding, we demonstrated that NmDsbA3 acts as a thiol-disulfide oxidoreductase in vitro and is reoxidized by Escherichia coli DsbB (EcDsbB). However, pronounced differences in the structures between DsbA3 and EcDsbA, which are clustered around the active site of the enzyme, suggested a structural basis for the unusual substrate specificity that is observed for NmDsbA3.

Structural and biochemical characterization of the oxidoreductase NmDsbA3 from Neisseria meningitidis.,Vivian JP, Scoullar J, Robertson AL, Bottomley SP, Horne J, Chin Y, Wielens J, Thompson PE, Velkov T, Piek S, Byres E, Beddoe T, Wilce MC, Kahler CM, Rossjohn J, Scanlon MJ J Biol Chem. 2008 Nov 21;283(47):32452-61. Epub 2008 Aug 20. PMID:18715864<ref>PMID:18715864</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2znm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Neisseria meningitidis serogroup B]]
[[Category: Beddoe T]]
[[Category: Bottomley SP]]
[[Category: Byres E]]
[[Category: Chin Y]]
[[Category: Horne J]]
[[Category: Kahler C]]
[[Category: Piek S]]
[[Category: Robertson AL]]
[[Category: Rossjohn J]]
[[Category: Scanlon MJ]]
[[Category: Scoullar J]]
[[Category: Thompson PE]]
[[Category: Velkov T]]
[[Category: Vivian JP]]
[[Category: Wielens J]]
[[Category: Wilce MCJ]]