View source for 2zwn


==Crystal structure of the novel two-domain type laccase from a metagenome==
<StructureSection load='2zwn' size='340' side='right'caption='[[2zwn]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2zwn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenomes Metagenomes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZWN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zwn OCA], [https://pdbe.org/2zwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zwn RCSB], [https://www.ebi.ac.uk/pdbsum/2zwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zwn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C0STU6_9BACT C0STU6_9BACT] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zw/2zwn_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zwn ConSurf].
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A multi-copper protein with two cupredoxin-like domains was identified from our in-house metagenomic database. The recombinant protein, mgLAC, contained four copper ions/subunits, oxidized various phenolic and non-phenolic substrates, and had spectroscopic properties similar to common laccases. X-ray structure analysis revealed a homotrimeric architecture for this enzyme, which resembles nitrite reductase (NIR). However, a difference in copper coordination was found at the domain interface. mgLAC contains a T2/T3 tri-nuclear copper cluster at this site, whereas a mononuclear T2 copper occupies this position in NIR. The trimer is thus an essential part of the architecture of two-domain multi-copper proteins, and mgLAC may be an evolutionary precursor of NIR.

X-ray structure of a two-domain type laccase: a missing link in the evolution of multi-copper proteins.,Komori H, Miyazaki K, Higuchi Y FEBS Lett. 2009 Apr 2;583(7):1189-95. Epub 2009 Mar 11. PMID:19285076<ref>PMID:19285076</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2zwn" style="background-color:#fffaf0;"></div>

==See Also==
*[[Laccase 3D structures|Laccase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Metagenomes]]
[[Category: Higuchi Y]]
[[Category: Komori H]]
[[Category: Miyazaki K]]