View source for 30gj


==W-formate dehydrogenase from Nitratidesulfovibrio vulgaris (Desulfovibrio vulgaris) - Ambient temperature, Serial crystallography==
<StructureSection load='30gj' size='340' side='right'caption='[[30gj]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[30gj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=30GJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=30GJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=W:TUNGSTEN+ION'>W</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=30gj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=30gj OCA], [https://pdbe.org/30gj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=30gj RCSB], [https://www.ebi.ac.uk/pdbsum/30gj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=30gj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q72EJ1_NITV2 Q72EJ1_NITV2] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Metal-dependent formate dehydrogenases (Fdhs) are a promising research target in efforts to mitigate climate change by developing active, efficient, selective and safe industrial catalysts for CO(2) reduction. Here, we report the room-temperature (RT) serial synchrotron X-ray crystallography (SSX) structure of Nitratidesulfovibrio vulgaris formate dehydrogenase AB and compare this structure with previously reported cryogenic structures. The comparison reveals structural differences likely arising from the absence of cryoprotectants, differences in data-collection temperature and the much lower radiation dose accumulated by each crystal during the SSX experiments. The RT-SSX structure provides the first step towards time-resolved serial crystallography experiments targeting possible catalytic intermediates in order to fully understand the catalytic mechanism of metal-dependent Fdhs.

Room-temperature crystal structure of a metal-dependent W-formate dehydrogenase by serial synchrotron crystallography.,Vilela-Alves G, Martins G, von Stetten D, Mehrabi P, Pereira IAC, Romao MJ, Pearson AR, Mota C Acta Crystallogr D Struct Biol. 2026 Sep 1. doi: 10.1107/S2059798326006923. PMID:42572941<ref>PMID:42572941</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 30gj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Desulfovibrio vulgaris str. Hildenborough]]
[[Category: Large Structures]]
[[Category: Martins G]]
[[Category: Mehrabi P]]
[[Category: Mota C]]
[[Category: Pearson AR]]
[[Category: Pereira IC]]
[[Category: Romao MJ]]
[[Category: Vilela-Alves G]]
[[Category: Von Stetten D]]