View source for 30ib


==NMR Solution Structure of Cold Shock Protein CspA==
<StructureSection load='30ib' size='340' side='right'caption='[[30ib]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[30ib]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=30IB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=30IB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR,  models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=30ib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=30ib OCA], [https://pdbe.org/30ib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=30ib RCSB], [https://www.ebi.ac.uk/pdbsum/30ib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=30ib ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CSPA_ECOLI CSPA_ECOLI] Binds to and stimulates the transcription of the CCAAT-containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3'.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cold-shock proteins (CSPs) are highly conserved nucleic acid-binding proteins that act as chaperones during cellular adaptation to low temperatures. Here, we present a comprehensive structural and dynamic characterization of Escherichia coli CspA using high-resolution NMR spectroscopy. The solution structure of CspA (PDB ID: 30IB) is supported by extensive NMR experimental restraints, minimal violations, and favorable stereochemistry, establishing it as a well-converged NMR structure. Crucially, we investigated backbone dynamics across multiple timescales, with a particular focus on the microsecond-millisecond regime using a combination of (15)N Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion and (15)N chemical exchange saturation transfer (CEST) experiments, together with visible peak-position constraints. To our knowledge, this represents the first application of such a combined (15)N CEST, (15)N CPMG and visible peak-position constraints approach to probe conformational exchange in CSPs. Our results show that, in addition to the conserved aromatic residues of RNP1 and RNP2 motifs that mediate pi-stacking interactions with nucleic acids, an unexpectedly broad network of hydrophobic core and solvent-exposed polar residues undergoes conformational exchange. Notably, residues in the beta3-beta4 and beta4-beta5 loops display complex dynamics not fully captured by model-free analysis formalism, highlighting their role in binding site flexibility. Complementary AF3/YASARA modeling of the CspA bound to heptathymidine (dT7) further supported that aromatic and polar residues form pi-stacking and ionic interactions with ssDNA bases, corroborating the functional relevance of these dynamic regions. Therefore, our findings demonstrate that CspA relies on a dynamic network extending from conserved motifs through the hydrophobic core and flexible loops, conferring the structural adaptability required for efficient nucleic acid recognition and chaperone activity.

Structural and functional insights into a mesophilic cold shock protein CspA with enhanced precision.,Wanko Nembot M, Feller G, Volkov AN, le Paige UB, Bouvignies G, Damblon C J Magn Reson. 2026 May 1;388:108077. doi: 10.1016/j.jmr.2026.108077. PMID:42102648<ref>PMID:42102648</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 30ib" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Dambon C]]
[[Category: Feller G]]
[[Category: Volkov O]]
[[Category: Wanko M]]