View source for 30sc


==Hairpin Inosine GCAA tetraloop==
<StructureSection load='30sc' size='340' side='right'caption='[[30sc]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[30sc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=30SC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=30SC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR,  models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=30sc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=30sc OCA], [https://pdbe.org/30sc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=30sc RCSB], [https://www.ebi.ac.uk/pdbsum/30sc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=30sc ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Synthetic RNAs bearing deazapurine nucleobases are powerful probes for dissecting RNA-catalyzed reactions by atomic mutagenesis. Here we systematically characterize RNA containing 1-deazainosine (c(1)I) and compare it with inosine (I). We first report the synthesis of a suitably protected c(1)I phosphoramidite and its incorporation into RNA by solid-phase synthesis. We then provide a comprehensive thermodynamic analysis of base-pair stability from UV-melting experiments, showing that c(1)I-C pairs are less stable than the corresponding I-C pairs. Although a two-hydrogen-bond Hoogsteen interaction between c(1)I and protonated C is conceivable, NMR spectroscopy indicates that c(1)I-C predominantly adopts a Watson-Crick-like geometry with a single hydrogen bond. These pairs are accommodated within RNA duplexes without disrupting neighboring base pairing. We also use c(1)I to probe poly(I:C) motifs that mimic viral double-stranded RNA, assessing how strand length governs duplex versus hairpin formation. Finally, atomic mutagenesis of the twister ribozyme with c(1)I supports the hypothesis that an active-site guanine participates directly in phosphodiester-bond cleavage. Together, these results clarify how deazapurines modulate nucleic-acid properties and provide guidance for their use in atomic mutagenesis to interrogate RNA catalysis.

1-Deazainosine-impact on RNA structure and role in exploring ribozyme catalysis.,Mitteregger C, Bereiter R, Schramm A, Ennifar E, Kreutz C, Micura R Chem Sci. 2026 Jun 8. doi: 10.1039/d6sc04009h. PMID:42266902<ref>PMID:42266902</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 30sc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Kreutz C]]
[[Category: Micura R]]
[[Category: Mitteregger C]]