View source for 3aj1


==The structure of AxCeSD octamer (N-terminal HIS-tag) from Acetobacter xylinum==
<StructureSection load='3aj1' size='340' side='right'caption='[[3aj1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3aj1]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"acetobacter_bordeaux"_(sic)_janke_1957 "acetobacter bordeaux" (sic) janke 1957]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2z9e 2z9e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AJ1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3AJ1 FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2z9e|2z9e]], [[3aj2|3aj2]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3aj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aj1 OCA], [http://pdbe.org/3aj1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3aj1 RCSB], [http://www.ebi.ac.uk/pdbsum/3aj1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3aj1 ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aj/3aj1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3aj1 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The cellulose synthesizing terminal complex consisting of subunits A, B, C, and D in Acetobacter xylinum spans the outer and inner cell membranes to synthesize and extrude glucan chains, which are assembled into subelementary fibrils and further into a ribbon. We determined the structures of subunit D (AxCeSD/AxBcsD) with both N- and C-terminal His(6) tags, and in complex with cellopentaose. The structure of AxCeSD shows an exquisite cylinder shape (height: approximately 65 A, outer diameter: approximately 90 A, and inner diameter: approximately 25 A) with a right-hand twisted dimer interface on the cylinder wall, formed by octamer as a functional unit. All N termini of the octamer are positioned inside the AxCeSD cylinder and create four passageways. The location of cellopentaoses in the complex structure suggests that four glucan chains are extruded individually through their own passageway along the dimer interface in a twisted manner. The complex structure also shows that the N-terminal loop, especially residue Lys6, seems to be important for cellulose production, as confirmed by in vivo assay using mutant cells with axcesD gene disruption and N-terminus truncation. Taking all results together, a model of the bacterial terminal complex is discussed.

Structure of bacterial cellulose synthase subunit D octamer with four inner passageways.,Hu SQ, Gao YG, Tajima K, Sunagawa N, Zhou Y, Kawano S, Fujiwara T, Yoda T, Shimura D, Satoh Y, Munekata M, Tanaka I, Yao M Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):17957-61. Epub 2010 Oct 4. PMID:20921370<ref>PMID:20921370</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3aj1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Hu, S Q]]
[[Category: Tajima, K]]
[[Category: Tanaka, I]]
[[Category: Yao, M]]
[[Category: Zhou, Y]]
[[Category: Alpha and beta fold]]
[[Category: Biosynthetic protein]]
[[Category: Cellulose biosynthesis]]
[[Category: Molecule ring]]
[[Category: National project on protein structural and functional analyse]]
[[Category: Nppsfa]]
[[Category: Octamer]]
[[Category: Structural genomic]]
[[Category: Tetramer of dimer]]