View source for 3ayl


==X-ray crystal structures of L-phenylalanine oxidase (deaminating and decaboxylating) from Pseudomonas sp. P501. Structures of the enzyme-ligand complex and catalytic mechanism==
<StructureSection load='3ayl' size='340' side='right'caption='[[3ayl]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ayl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._P-501 Pseudomonas sp. P-501]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AYL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ayl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ayl OCA], [https://pdbe.org/3ayl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ayl RCSB], [https://www.ebi.ac.uk/pdbsum/3ayl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ayl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PAO_PSESP PAO_PSESP] Catalyzes both oxygenative decarboxylation and oxidative deamination, depending on the substrate used. Has high activity for L-Phe and L-Tyr, but relatively low activities for L-Met and L-Trp. L-Phe is mainly oxygenated and L-Met is mainly oxidized.<ref>PMID:15632301</ref> <ref>PMID:16567420</ref> <ref>PMID:3818566</ref> <ref>PMID:6501250</ref> <ref>PMID:6885723</ref> <ref>PMID:7174643</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The mature form of l-Phe oxidase of Pseudomonas sp. P-501 (PAOpt) catalyzes the oxygenative decarboxylation of l-Phe and the oxidative deamination of l-Met, and is highly specific for l-Phe. The crystal structures of PAOpt individually complexed with l-Phe and l-Met and the properties of the active site mutants were investigated to clarify the structural basis of the substrate and reaction specificities of the enzyme. The benzene ring of l-Phe is packed in 6 hydrophobic amino acid side chains versus the 2 hydrophobic side chains of l-amino acid oxidase (LAO, pdb code: 2jb2); the distance between the substrate Calpha atom and water is shorter in the PAOpt- l-Met complex than in the PAOpt-l-Phe complex; and the mutation of substrate carboxylate-binding residues (Arg143 and Tyr536) causes the enzyme to oxidize l-Phe and decreases the charge-transfer band with l-Phe. These results suggest that (1) the higher substrate specificity of PAOpt relative to LAO is derived from the compact hydrophobic nature of the PAOpt active site, and (2) the reactivity of the PAOpt charge-transfer complex with water or oxygen determines whether the enzyme catalyzes oxidation or oxygenation, respectively.

High resolution X-ray crystal structures of L-phenylalanine oxidase (deaminating and decarboxylating) from Pseudomonas sp. P-501. Structures of the enzyme-ligand complex and catalytic mechanism.,Ida K, Suguro M, Suzuki H J Biochem. 2011 Aug 13. PMID:21841183<ref>PMID:21841183</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3ayl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas sp. P-501]]
[[Category: Suzuki H]]