View source for 3ays


==GH5 endoglucanase from a ruminal fungus in complex with cellotriose==
<StructureSection load='3ays' size='340' side='right'caption='[[3ays]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ays]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Piromyces_rhizinflatus Piromyces rhizinflatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AYS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PRD_900021:beta-cellotriose'>PRD_900021</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ays FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ays OCA], [https://pdbe.org/3ays PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ays RCSB], [https://www.ebi.ac.uk/pdbsum/3ays PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ays ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9URH5_9FUNG Q9URH5_9FUNG] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The endoglucanase EglA from Piromyces rhizinflata found in cattle stomach belongs to the GH5 family of glycoside hydrolases. The crystal structure of the catalytic domain of EglA shows the (beta/alpha)(8)-barrel fold typical of GH5 enzymes. Adjacent to the active site of EglA, a loop containing a disulfide bond not found in other similar structures may participate in substrate binding. Because the active site was blocked by the N-terminal His tag of a neighbouring protein molecule in the crystal, enzyme-substrate complexes could not be obtained by soaking but were prepared by cocrystallization. The E154A mutant structure with a cellotriose bound to the -3, -2 and -1 subsites shows an extensive hydrogen-bonding network between the enzyme and the substrate, along with a stacking interaction between Trp44 and the -3 sugar. A possible dimer was observed in the crystal structure, but retention of activity in the E242A mutant suggested that the enzyme probably does not function as a dimer in solution. On the other hand, the first 100 amino acids encoded by the original cDNA fragment are very similar to those in the last third of the (beta/alpha)(8)-barrel fold, indicating that EglA comprises at least two catalytic domains acting in tandem.

Substrate binding of a GH5 endoglucanase from the ruminal fungus Piromyces rhizinflata.,Tseng CW, Ko TP, Guo RT, Huang JW, Wang HC, Huang CH, Cheng YS, Wang AH, Liu JR Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1189-94., Epub 2011 Sep 24. PMID:22102024<ref>PMID:22102024</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3ays" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Piromyces rhizinflatus]]
[[Category: Guo R-T]]
[[Category: Ko T-P]]
[[Category: Liu J-R]]
[[Category: Tseng C-W]]