View source for 3bw8


==Crystal structure of the Clostridium limosum C3 exoenzyme==
<StructureSection load='3bw8' size='340' side='right'caption='[[3bw8]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3bw8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hathewaya_limosa Hathewaya limosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BW8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bw8 OCA], [https://pdbe.org/3bw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bw8 RCSB], [https://www.ebi.ac.uk/pdbsum/3bw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bw8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARC3_HATLI ARC3_HATLI] ADP-ribosylates eukaryotic Rho and Rac proteins on an asparagine residue.[UniProtKB:P15879]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bw8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bw8 ConSurf].
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== Publication Abstract from PubMed ==
C3-like toxins ADP-ribosylate and inactivate Rho GTPases. Seven C3-like ADP-ribosyltransferases produced by Clostridium botulinum, Clostridium limosum, Bacillus cereus and Staphylococcus aureus were identified and two representatives--C3bot from C. botulinum and C3stau2 from S. aureus--were crystallized. Here we present the 1.8A structure of C. limosum C3 transferase C3lim and compare it to the structures of other family members. In contrast to the structure of apo-C3bot, the canonical ADP-ribosylating turn turn motif is observed in a primed conformation, ready for NAD binding. This suggests an impact on the binding mode of NAD and on the transferase reaction. The crystal structure explains why auto-ADP-ribosylation of C3lim at Arg41 interferes with the ADP-ribosyltransferase activity of the toxin.

Crystal structure of the Clostridium limosum C3 exoenzyme.,Vogelsgesang M, Stieglitz B, Herrmann C, Pautsch A, Aktories K FEBS Lett. 2008 Apr 2;582(7):1032-6. Epub 2008 Mar 4. PMID:18325337<ref>PMID:18325337</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3bw8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Exoenzyme 3D structures|Exoenzyme 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hathewaya limosa]]
[[Category: Large Structures]]
[[Category: Aktories K]]
[[Category: Herrmann C]]
[[Category: Pautsch A]]
[[Category: Stieglitz B]]
[[Category: Vogelsgesang M]]