View source for 3frl


==The 2.25 A crystal structure of LipL32, the major surface antigen of Leptospira interrogans serovar Copenhageni==
<StructureSection load='3frl' size='340' side='right'caption='[[3frl]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3frl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_interrogans_serovar_Copenhageni Leptospira interrogans serovar Copenhageni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FRL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FRL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=211:2,2,2-NITRILOTRIETHANOL'>211</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3frl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3frl OCA], [https://pdbe.org/3frl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3frl RCSB], [https://www.ebi.ac.uk/pdbsum/3frl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3frl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q72SM7_LEPIC Q72SM7_LEPIC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Leptospirosis, a spirochaetal zoonotic disease caused by Leptospira, has been recognized as an important emerging infectious disease. LipL32 is the major exposed outer membrane protein found exclusively in pathogenic leptospires, where it accounts for up to 75% of the total outer membrane proteins. It is highly immunogenic, and recent studies have implicated LipL32 as an extracellular matrix binding protein, interacting with collagens, fibronectin, and laminin. In order to better understand the biological role and the structural requirements for the function of this important lipoprotein, we have determined the 2.25-A-resolution structure of recombinant LipL32 protein corresponding to residues 21-272 of the wild-type protein (LipL32(21-272)). The LipL32(21-272) monomer is made of a jelly-roll fold core from which several peripheral secondary structures protrude. LipL32(21-272) is structurally similar to several other jelly-roll proteins, some of which bind calcium ions and extracellular matrix proteins. Indeed, spectroscopic data (circular dichroism, intrinsic tryptophan fluorescence, and extrinsic 1-amino-2-naphthol-4-sulfonic acid fluorescence) confirmed the calcium-binding properties of LipL32(21-272). Ca(2+) binding resulted in a significant increase in the thermal stability of the protein, and binding was specific for Ca(2+) as no structural or stability perturbations were observed for Mg(2+), Zn(2+), or Cu(2+). Careful examination of the crystallographic structure suggests the locations of putative regions that could mediate Ca(2+) binding as well as binding to other interacting host proteins, such as collagens, fibronectin, and laminin.

Structure and calcium-binding activity of LipL32, the major surface antigen of pathogenic Leptospira sp.,Hauk P, Guzzo CR, Roman Ramos H, Ho PL, Farah CS J Mol Biol. 2009 Jul 24;390(4):722-36. Epub 2009 May 25. PMID:19477185<ref>PMID:19477185</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3frl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Leptospira interrogans serovar Copenhageni]]
[[Category: Farah CS]]
[[Category: Guzzo CR]]
[[Category: Hauk P]]
[[Category: Ho PL]]