View source for 3iaj


==Crystal structure of a betagamma-crystallin domain from Clostridium beijerinckii-in alternate space group I422==
<StructureSection load='3iaj' size='340' side='right'caption='[[3iaj]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3iaj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_beijerinckii_NCIMB_8052 Clostridium beijerinckii NCIMB 8052]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IAJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iaj OCA], [https://pdbe.org/3iaj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iaj RCSB], [https://www.ebi.ac.uk/pdbsum/3iaj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iaj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A6LX94_CLOB8 A6LX94_CLOB8] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/3iaj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iaj ConSurf].
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== Publication Abstract from PubMed ==
The betagamma-crystallin superfamily consists of evolutionarily related proteins with domain topology similar to lens beta- and gamma-crystallins, formed from duplicated Greek key motifs. Ca2+-binding was found in a few betagamma-crystallin members earlier, although its prevalence and diversity as an inherent molecular property among members of the superfamily is not well-studied. To increase our understanding of Ca2+-binding in various betagamma-crystallins, we undertook comprehensive structural and Ca2+-binding studies of seven members of the superfamily from bacteria, archaea and vertebrates, including determination of high resolution crystal structures of three proteins. Our structural observations show that the determinants of Ca2+ coordination remain conserved in the form of an N/D-N/D-#-I-S/T-S motif in all domains. However, binding of Ca2+ elicits varied physico-chemical responses, ranging from passive sequestration to active stabilization. The motif in this superfamily is modified in some members like lens crystallins where Ca2+-binding abilities are partly or completely compromised. We show that reduction or loss of Ca2+-binding in members of the superfamily, particularly in vertebrates, is due to the selective presence of unfavorable amino acids (largely Arg) at key Ca2+-ligation positions and that engineering of the canonical Ca2+-binding residues can confer binding activity on an otherwise inactive domain. Through this work, we demonstrate that betagamma-crystallins with the N/D-N/D-#-I-S/T-S motif form an extensive set of Ca2+-binding proteins prevalent in all the three kingdoms of life.

betagamma-Crystallin superfamily contains a universal motif for binding calcium.,Aravind P, Mishra A, Suman SK, Jobby MK, Sankaranarayanan R, Sharma Y Biochemistry. 2009 Nov 18. PMID:19921810<ref>PMID:19921810</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3iaj" style="background-color:#fffaf0;"></div>

==See Also==
*[[Crystallin 3D structures|Crystallin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Clostridium beijerinckii NCIMB 8052]]
[[Category: Large Structures]]
[[Category: Aravind P]]
[[Category: Sankaranarayanan R]]