View source for 3ifi


==2'-SeMe-dG modified octamer DNA==
<StructureSection load='3ifi' size='340' side='right'caption='[[3ifi]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ifi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IFI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XUG:2-SE-METHYL-2-SELENOGUANOSINE+5-(DIHYDROGEN+PHOSPHATE)'>XUG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ifi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ifi OCA], [https://pdbe.org/3ifi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ifi RCSB], [https://www.ebi.ac.uk/pdbsum/3ifi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ifi ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Selenium modification of nucleic acids is of great importance in X-ray crystal structure determination and functional study of nucleic acids. Herein, we describe a convenient synthesis of a new building block, the 2'-SeMe-modified guanosine (G(Se)) phosphoramidite, and report the first incorporation of the 2'-Se-G moiety into DNA. The X-ray crystal structure of the 2'-Se-modified octamer DNA (5'-GTG(Se)TACAC-3') was determined at a resolution of 1.20 A. We also found that the 2'-Se modification points to the minor groove and that the modified and native structures are virtually identical. Furthermore, we observed that the 2'-Se-G modification can significantly facilitate the crystal growth with respect to the corresponding native DNA.

Synthesis and crystal structure of 2'-se-modified guanosine containing DNA.,Salon J, Sheng J, Gan J, Huang Z J Org Chem. 2010 Feb 5;75(3):637-41. PMID:20047333<ref>PMID:20047333</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3ifi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Gan J]]
[[Category: Huang Z]]
[[Category: Salon J]]
[[Category: Sheng J]]