View source for 3j7r


==Structure of the translating mammalian ribosome-Sec61 complex==
<SX load='3j7r' size='340' side='right' viewer='molstar' caption='[[3j7r]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3j7r]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4w26 4w26], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4w27 4w27], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4w28 4w28], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx0 1vx0], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx1 1vx1], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx2 1vx2] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j73 3j73]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J7R FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7r OCA], [https://pdbe.org/3j7r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j7r RCSB], [https://www.ebi.ac.uk/pdbsum/3j7r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7r ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F1SJJ5_PIG F1SJJ5_PIG] Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.[ARBA:ARBA00034092]
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== Publication Abstract from PubMed ==
Cotranslational protein translocation is a universally conserved process for secretory and membrane protein biosynthesis. Nascent polypeptides emerging from a translating ribosome are either transported across or inserted into the membrane via the ribosome-bound Sec61 channel. Here, we report structures of a mammalian ribosome-Sec61 complex in both idle and translating states, determined to 3.4 and 3.9 A resolution. The data sets permit building of a near-complete atomic model of the mammalian ribosome, visualization of A/P and P/E hybrid-state tRNAs, and analysis of a nascent polypeptide in the exit tunnel. Unprecedented chemical detail is observed for both the ribosome-Sec61 interaction and the conformational state of Sec61 upon ribosome binding. Comparison of the maps from idle and translating complexes suggests how conformational changes to the Sec61 channel could facilitate translocation of a secreted polypeptide. The high-resolution structure of the mammalian ribosome-Sec61 complex provides a valuable reference for future functional and structural studies.

Structure of the Mammalian ribosome-sec61 complex to 3.4 a resolution.,Voorhees RM, Fernandez IS, Scheres SH, Hegde RS Cell. 2014 Jun 19;157(7):1632-43. doi: 10.1016/j.cell.2014.05.024. Epub 2014 Jun , 12. PMID:24930395<ref>PMID:24930395</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3j7r" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Fernandez IS]]
[[Category: Hegde RS]]
[[Category: Scheres SHW]]
[[Category: Voorhees RM]]